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Review
. 2017 Nov-Dec;14(6):461-467.
doi: 10.21873/cgp.20056.

DHPLC Elution Patterns of VDR PCR Products Can Predict Prostate Cancer Susceptibility in African American Men

Robert L Copeland  1   2 , Desta Beyene  2 , Victor Apprey  3 , Mohammad R Daremipouran  2 , Tammey J Naab  4 , Olakunle O Kassim  5 , Yasmine M Kanaan  6   5
Affiliations
Review

DHPLC Elution Patterns of VDR PCR Products Can Predict Prostate Cancer Susceptibility in African American Men

Robert L Copeland et al. Cancer Genomics Proteomics. 2017 Nov-Dec.

Abstract

Background/aim: Denaturing high-performance liquid chromatography (DHPLC) is a technique that is used to detect mutations. The aim of the present study was to determine whether DHPLC elution patterns of vitamin D receptor (VDR) gene PCR products can serve as indicators of susceptibility to prostate cancer (PCa) risk.

Materials and methods: DNA samples of PCa cases and controls were screened for mutations and/or polymorphisms in coding exons of VDR gene using DHPLC analysis. Logistic regression, phi-coefficient (ϕ), and Backward Wald models were used to analyze the data.

Results: Similar elution patterns of exons 1, 6, 7 and 9 along with higher prevalence of heteroduplex DNA were observed in PCa samples than in controls. Exons 4 and 8 had highly significant protective effects (p<0.05). Whereas, exons 5, 7, and 9 were perfectly positively correlated with PCa risk (ϕ=1), thus presenting candidate exons significantly associated with susceptibility to PCa.

Conclusion: DHPLC elution patterns of the selected exons could be useful to predict susceptibility to develop PCa.

Keywords: African American men; Prostate cancer; vitamin D; vitamin D receptor gene.

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