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. 2017 Nov 6;7(1):14551.
doi: 10.1038/s41598-017-15061-w.

The comprehensive expression analysis of circular RNAs in gastric cancer and its association with field cancerization

Amanda Ferreira Vidal  1 , André M Ribeiro-Dos-Santos  1 , Tatiana Vinasco-Sandoval  1 , Leandro Magalhães  1 , Pablo Pinto  1 , Ana K M Anaissi  2 , Samia Demachki  2 , Paulo Pimentel de Assumpção  2 , Sidney Emanuel Batista Dos Santos  1   2 , Ândrea Ribeiro-Dos-Santos  3   4
Affiliations

The comprehensive expression analysis of circular RNAs in gastric cancer and its association with field cancerization

Amanda Ferreira Vidal et al. Sci Rep. .

Abstract

Circular RNAs comprise a new class of long noncoding RNAs characterized by their 5' and 3' ends covalently joined. Previous studies have demonstrated that some circular RNAs act as microRNA sponges, and are associated with cellular proliferation in cancer. We were the first to analyze the global expression of circular RNAs in samples of patients without gastric cancer, gastric cancer, and matched tumor-adjacent gastric tissue. Among the samples, we identified 736 previously annotated circular RNAs by RNA-Seq. The tumor-adjacent tissue presented the higher abundance of circular RNAs and could not be considered as a normal tissue, reinforcing the notion of field effect in gastric cancer. We identified five differentially expressed circular RNAs that may be potential biomarkers of this type of cancer. We also predicted candidate microRNAs targets of the highest expressed circular RNAs in gastric tissues and found five miRNAs. Overall, our results support the hypothesis of circular RNAs representing a novel factor in the dynamic epigenetic network of gene regulation, which involves the microRNAs, its mRNAs targets, and the circular RNAs-derived genes. Further studies are needed to elucidate the roles and the functional relevance of the circular RNAs in human diseases.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Total of annotated circular RNAs detected in gastric tissue. (a) Number of expressed circular RNAs in each type of gastric tissue according to their origin. (b) Venn diagram of all expressed circular RNAs between the three types of gastric tissue. CDS: coding DNA sequence.
Figure 2
Figure 2
GO enrichment of the gastric circular RNAs-derived genes, evidencing the KEGG pathways and its scores. (A) Gastric tissue without gastric cancer. (B) Matched tumor-adjacent gastric tissue. (C) Gastric cancer.
Figure 3
Figure 3
Simulation of the relation between CORO1C, hsa_circ_0000437 and hsa-miR-1. Pol II: RNA polymerase II.
Figure 4
Figure 4
Expression of the five differentially expressed circular RNAs in gastric tissue. This analysis was performed by comparing the samples without cancer with both tumor-adjacent and gastric cancer samples combined.
See this image and copyright information in PMC

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