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. 2017 Nov;14(5):5865-5870.
doi: 10.3892/ol.2017.6924. Epub 2017 Sep 13.

The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis

Hai Li  1 , Zhenwei Qiu  1 , Feng Li  2 , Chunlei Wang  2
Affiliations

The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis

Hai Li et al. Oncol Lett. 2017 Nov.

Abstract

The relationship between the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 and breast cancer prognosis was studied. Two breast cancer cell lines (MDA-MB-231 and MCF-7) and one human normal breast cell line (HS578Bst) were investigated. Fluorescence real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect cellular mRNA and protein MMP-2 and MMP-9 expression levels. Breast cancer tissue samples from 80 patients and tumor-adjacent normal tissue samples from 40 patients were collected, and MMP-2 and MMP-9 expression in these samples were examined using immunohistochemistry (IHC). The relationship of MMP-2 and MMP-9 expression levels with breast cancer patient clinicopathological parameters and prognosis was analyzed. RT-PCR and western blot results showed that MMP-2 and MMP-9 mRNA and protein expression levels were significantly higher in MDA-MB-231 and MCF-7 cells than in HS578Bst cells. A high expression of MMP-2 and MMP-9 was found in 83.75% (67/80) and 78.75% (63/80) of breast cancer tissue samples, respectively. MMP-2 and MMP-9 expression in breast cancer tissues were significantly different from that in tumor-adjacent normal tissues (p<0.01). MMP-2 and MMP-9 expression levels in breast cancer tissues were correlated with lymph node metastasis and tumor staging. Single factor survival analysis showed that MMP-2 and MMP-9 were factors influencing breast cancer prognosis. MMP-2 and MMP-9 are highly expressed in breast cancer tissues and are closely related to lymph node metastasis and tumor staging. MMP-2 and MMP-9 can be used as reference indices for guiding breast cancer prognosis and treatment.

Keywords: breast cancer; matrix metalloproteinase-2; matrix metalloproteinase-9; prognosis.

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Figures

Figure 1.
Figure 1.
Detection of matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA expression in MCF-7, MDA-MB-231 and HS578Bst cells. The expression levels of MMP-2 and MMP-9 mRNA in MDA-MB-231 and MCF-7 cells were significantly higher than in HS578Bst cells, **p<0.01.
Figure 2.
Figure 2.
(A and B) Detection of matrix metalloproteinase-2 (MMP-2) and MMP-9 protein expression in MCF-7, MDA-MB-231 and HS578Bst cells by western blot analysis (**p<0.05).
Figure 3.
Figure 3.
Detection of matrix metalloproteinase-2 (MMP-2) and MMP-9 protein expression in clinicopathological tissues via immunohistochemistry (IHC) MMP-2 and MMP-9 are expressed in the cytoplasm and appear as brown granules; a proportion of positive cells >25% in the visual field represented high expression, otherwise, the result was categorized as low expression (×400).
Figure 4.
Figure 4.
(A and B) Survival curves for patients with high and low expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 Kaplan-Meier analysis shows that patients with low expression of MMP-2 and MMP-9 showed improved prognoses.
See this image and copyright information in PMC

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