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Comment
. 2017 Nov 21;114(47):12362-12364.
doi: 10.1073/pnas.1717798114. Epub 2017 Nov 7.

Haplotype resolution at the single-cell level

Andrew C Adey  1   2
Affiliations
Comment

Haplotype resolution at the single-cell level

Andrew C Adey. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
(A) Common challenges of identifying single-nucleotide variants (SNVs) from single-cell genome sequencing data. Failure of individual strands to amplify can result in allelic dropout and false homozygous (Hom.) calls. Even when both alleles are amplified, the haplotype (Hap) assignment is not possible. Lastly, errors can look identical to heterozygous (Het.) SNVs and cannot be detected. (B) Diluting individual strands into separate amplification compartments enables the association of variants to the same haplotype (haplotype phasing; dark loops at the top indicate the association of heterozygous variants to a haplotype), as well as the detection of errors when only one of the four amplified strands harbors a given variant.
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