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. 2018:1701:381-391.
doi: 10.1007/978-1-4939-7447-4_21.

Antibody Selection on FFPE Tissue Slides

Andre Ten Haaf  1 , Stefan Gattenlöhner  2 , Mehmet Kemal Tur  3
Affiliations

Antibody Selection on FFPE Tissue Slides

Andre Ten Haaf et al. Methods Mol Biol. 2018.

Abstract

Standard antibody phage-display panning uses purified proteins, antigen-transfected cells, or tumor cell lines as target structure to generate specific antibodies. Here, we describe a method for the selection of specific antibodies by phage panning against routine formalin-fixed paraffin-embedded (FFPE) tissue biopsies immobilized on glass slides. Selected antibody fragments recognize disease-associated antigens in its native conformation, suitable for the development of targeted diagnostic and therapeutic agents.

Keywords: Formalin-fixed paraffin-embedded (FFPE) tissue-specific antibodies; On-slide selection; Phage-display technology.

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