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. 2018:1788:145-156.
doi: 10.1007/7651_2017_93.

LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers

Yuqian Gao  1 , Hui Wang  1 , Carrie D Nicora  1 , Tujin Shi  1 , Richard D Smith  1 , Tara K Sigdel  2 , Minnie M Sarwal  2 , David G Camp 2nd  1 , Wei-Jun Qian  3
Affiliations

LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers

Yuqian Gao et al. Methods Mol Biol. 2018.

Abstract

Liquid chromatography (LC)-selected reaction monitoring (SRM) is a powerful protein quantification technique in terms of sensitivity, reproducibility, and multiplexing capability. LC-SRM can accurately measure the concentrations of surrogate proteotypic peptides for targeted proteins in complex biological samples by using their stable heavy isotope-labeled counterparts as internal standards. Herein, we describe a step-by-step protocol of the application of LC-SRM to quantify candidate protein biomarkers in human urine.

Keywords: Biomarker; LC-SRM; Skyline; Stable heavy isotope-labeled peptide; Targeted quantification; Urine.

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Figures

Fig. 1
Fig. 1
Workflow of LC-SRM quantification of candidate urinary protein biomarkers
Fig. 2
Fig. 2
Extracted ion chromatograms (XICs) of three peptides (A, B, C) in a urine sample. The dotted lines demonstrate the peak boundaries, while the arrows indicate the retention times. Peptide A shows confident detection and quantification, while peptide B lacks of clear signals, and peptide C suffers from matrix interferences
See this image and copyright information in PMC

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