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. 2017 Nov 9;7(1):15119.
doi: 10.1038/s41598-017-15156-4.

Association of long noncoding RNAs expression levels and their gene polymorphisms with systemic lupus erythematosus

Jun Li  1   2   3 , Guo-Cui Wu  1   2 , Tian-Ping Zhang  1   2 , Xiao-Ke Yang  1   2 , Shuang-Shuang Chen  1   2 , Lian-Ju Li  1   2 , Shu-Zhen Xu  1   2 , Tian-Tian Lv  1   2 , Rui-Xue Leng  1   2 , Hai-Feng Pan  4   5 , Dong-Qing Ye  6   7
Affiliations

Association of long noncoding RNAs expression levels and their gene polymorphisms with systemic lupus erythematosus

Jun Li et al. Sci Rep. .

Abstract

Increasing evidence has demonstrated the association between long noncoding RNAs (lncRNAs) and multiple autoimmune diseases. To explore four lncRNAs (GAS5, lnc-DC, linc0597 and linc0949) expression levels and gene polymorphisms in systemic lupus erythematosus (SLE), a two stage design was applied. In the first stage, 85 SLE patients and 71 healthy controls were enrolled to investigate the lncRNAs expression levels. Then, 1260 SLE patients and 1231 healthy controls were included to detect the single nucleotide polymorphisms (SNPs) in the differentially expressed lncRNAs identified in the first stage. Linc0597, lnc-DC and GAS5 expression levels were significantly lower in SLE patients than healthy controls (P < 0.001, P < 0.001, P = 0.003 respectively). Association of five SNPs (rs10515177, rs2070107, rs2632516, rs2877877, rs2067079) with SLE risk were analyzed. No significant association was observed between these gene polymorphisms and susceptibility to SLE (all P > 0.010), and we did not find significant association between any genotypes at five SNPs and their respective lncRNAs expression in SLE (all P > 0.010). In summary, the expression levels of linc0597, lnc-DC and GAS5 are decreased in SLE patients, but their gene polymorphisms are not associated with SLE risk, and do not influence their expression levels.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Comparison of expression of lncRNAs between different groups. Each symbol represents an individual subjects; horizontal lines indicate median values. The expression levels of the lncRNAs in 85 SLE patients, 71 healthy controls were analyzed by qRT-PCR and normalized by β-actin. (A) Decreased expression of linc0597 in patients with SLE versus healthy controls. (B) The expression of linc0949 in SLE and healthy controls did not show any difference. (C) The expression of lnc-DC in SLE was significantly lower than healthy controls. (D) The expression of GAS5 in SLE was significantly lower than healthy controls.
Figure 2
Figure 2
Comparison of expression of lncRNAs between lupus nephritis (LN) group and SLE non-LN group. (A) The expression levels of lin0597 in LN compared with non-LN. (B) The expression levels of lin0949 in LN compared with non-LN. (C) The expression levels of lnc-DC in LN compared with non-LN. (D) The expression levels of GAS5 in LN compared with non-LN.
See this image and copyright information in PMC

References

    1. Tsokos GC. Systemic lupus erythematosus. N Engl J Med. 2011;365:2110–21. doi: 10.1056/NEJMra1100359. - DOI - PubMed
    1. Guo J, et al. The association of novel IL-33 polymorphisms with sIL-33 and risk of systemic lupus erythematosus. Mol Immunol. 2016;77:1–7. doi: 10.1016/j.molimm.2016.07.001. - DOI - PubMed
    1. Leng RX, et al. Evidence for genetic association of TBX21 and IFNG with systemic lupus erythematosus in a Chinese Han population. Sci Rep. 2016;6:22081. doi: 10.1038/srep22081. - DOI - PMC - PubMed
    1. Chuang, H. C. et al. Downregulation of the phosphatase JKAP/DUSP22 in T Cells as a potential new biomarker of systemic lupus erythematosus nephritis. Oncotarget (2016). - PMC - PubMed
    1. Brownlie RJ, Zamoyska R. T cell receptor signalling networks: branched, diversified and bounded. Nat Rev Immunol. 2013;13:257–69. doi: 10.1038/nri3403. - DOI - PubMed

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