Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:6947376.
doi: 10.1155/2017/6947376. Epub 2017 Oct 19.

Influence of Digestion Procedure and Residual Carbon on Manganese, Copper, and Zinc Determination in Herbal Matrices by Atomic Absorption Spectrometry

Dorota Adamczyk-Szabela  1 , Piotr Anielak  1 , Wojciech M Wolf  1
Affiliations

Influence of Digestion Procedure and Residual Carbon on Manganese, Copper, and Zinc Determination in Herbal Matrices by Atomic Absorption Spectrometry

Dorota Adamczyk-Szabela et al. J Anal Methods Chem. 2017.

Abstract

Mineralization to the complete oxidation of sample carbon component does not always assure the best analyte recovery. Particular attention should be paid to the presence of silicon in the investigated plant sample and especially in the certified reference material for which Si content is scarcely given by the providers. During mineralization without addition of the hydrofluoric acid, the residual carbon may block silica surfaces and increase availability of an analyte for its spectral determination in the solution. This issue is of particular relevance because standard protocols for digestion of plant matrices often do not support hydrofluoric acid addition. Several procedures recommended for decomposition of herbal plants were applied for the respective certified reference material and examined in detail. Manganese, copper, and zinc contents were analyzed in all samples by the flame atomic absorption spectrometry. Additionally, the residual carbon was determined in all mineralizates. Silicon content was analyzed by the X-ray fluorescence method. The best recoveries were observed for samples characterized by relatively high residual carbon.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Recoveries versus NPOC as determined for all protocols which were applied for microwave digestion in a closed system: (a) Mn, (b) Zn, and (c) Cu.
Figure 2
Figure 2
Results of the one-way ANOVA calculations for manganese (a); zinc (b); copper (c) content as compared for all pairs of investigated methodologies. Grey squares represent combinations for which average concentrations are equal at the 0.95 probability level. Numerical values are given in the supplementary materials.
See this image and copyright information in PMC

References

    1. Baranowska I., Srogi K., Wlochowicz A., Szczepanik K. Determination of heavy metal contents in samples of medicinal herbs. Polish Journal of Environmental Studies. 2002;11(5):467–471.
    1. Demirel S., Tuzen M., Saracoglu S., Soylak M. Evaluation of various digestion procedures for trace element contents of some food materials. Journal of Hazardous Materials. 2008;152(3):1020–1026. doi: 10.1016/j.jhazmat.2007.07.077. - DOI - PubMed
    1. Bou R., Guardiola F., Padró A., Pelfort E., Codony R. Validation of mineralisation procedures for the determination of selenium, zinc, iron and copper in chicken meat and feed samples by ICP-AES and ICP-MS. Journal of Analytical Atomic Spectrometry. 2004;19(10):1361–1369. doi: 10.1039/B404558K. - DOI
    1. Hoenig M., Baeten H., Vanhentenrijk S., Vassileva E., Quevauviller P. Critical discussion on the need for an efficient mineralization procedure for the analysis of plant material by atomic spectrometric methods. Analytica Chimica Acta. 1998;358(1):85–94. doi: 10.1016/S0003-2670(97)00594-1. - DOI
    1. Hoenig M. Preparation steps in environmental trace element analysis — facts and traps. Talanta. 54(6):1021–1038. doi: 10.1016/S0039-9140(01)00329-0. - DOI - PubMed

LinkOut - more resources