doi: 10.1016/s0006-291x(89)80179-2.
Antibody-antigen binding in organic solvents
Affiliations
- PMID: 2912456
- DOI: 10.1016/s0006-291x(89)80179-2
Item in Clipboard
Antibody-antigen binding in organic solvents
Biochem Biophys Res Commun.
.
Abstract
We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.
Similar articles
-
PEGylated antibody in organic media.J Biosci Bioeng. 2011 May;111(5):564-8. doi: 10.1016/j.jbiosc.2011.01.001. Epub 2011 Feb 1. J Biosci Bioeng. 2011. PMID: 21277826
-
Dye-hydrophobic hapten conjugate/anti-dye antibody complex as immunogen: preparation of hydrophobic hapten-specific monoclonal antibodies.Biochimie. 1994;76(2):171-9. doi: 10.1016/0300-9084(94)90010-8. Biochimie. 1994. PMID: 8043652
-
Water immiscible solvent based immunoassay.J Immunoassay. 1997 Aug;18(3):235-46. doi: 10.1080/01971529708005817. J Immunoassay. 1997. PMID: 9257263
-
Studies on affinity constants of hapten-specific monoclonal antibodies using an antibody-immobilized ELISA.Biotechnol Prog. 1995 May-Jun;11(3):352-6. doi: 10.1021/bp00033a016. Biotechnol Prog. 1995. PMID: 7619405
-
The nature of the antigen-antibody bond and the factors affecting its association and dissociation.CRC Crit Rev Immunol. 1986;6(1):1-46. CRC Crit Rev Immunol. 1986. PMID: 3522103 Review.
Cited by
-
Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize.F1000Res. 2019 Jul 10;8:1042. doi: 10.12688/f1000research.19643.2. eCollection 2019. F1000Res. 2019. PMID: 31956398 Free PMC article.
-
Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles.Sensors (Basel). 2014 Nov 18;14(11):21843-57. doi: 10.3390/s141121843. Sensors (Basel). 2014. PMID: 25412219 Free PMC article.
-
5G-enabled ultra-sensitive fluorescence sensor for proactive prognosis of COVID-19.Biosens Bioelectron. 2021 Jun 1;181:113160. doi: 10.1016/j.bios.2021.113160. Epub 2021 Mar 13. Biosens Bioelectron. 2021. PMID: 33740542 Free PMC article.
-
Enzyme crystal structure in a neat organic solvent.Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8653-7. doi: 10.1073/pnas.90.18.8653. Proc Natl Acad Sci U S A. 1993. PMID: 8378343 Free PMC article.
-
Aspects of antibody-catalyzed primary amide hydrolysis.Appl Biochem Biotechnol. 1994 May-Jun;47(2-3):277-90; discussion 291-2. doi: 10.1007/BF02787940. Appl Biochem Biotechnol. 1994. PMID: 7944343
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
