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. 1989 Jan 16;158(1):80-5.
doi: 10.1016/s0006-291x(89)80179-2.

Antibody-antigen binding in organic solvents

A J Russell  1 , L J Trudel, P L Skipper, J D Groopman, S R Tannenbaum, A M Klibanov
Affiliations

Antibody-antigen binding in organic solvents

A J Russell et al. Biochem Biophys Res Commun. .

Abstract

We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.

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