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. 2018:1649:95-110.
doi: 10.1007/978-1-4939-7213-5_6.

LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing

Susanne Nichterwitz  1 , Julio Aguila Benitez  1   2 , Rein Hoogstraaten  1   3 , Qiaolin Deng  2 , Eva Hedlund  4
Affiliations

LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing

Susanne Nichterwitz et al. Methods Mol Biol. 2018.

Abstract

LCM-seq couples laser capture microdissection of cells from frozen tissues with polyA-based RNA sequencing and is applicable to single neurons. The method utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the positional information of each cell is preserved.

Keywords: Cresyl violet; Dopamine neuron; HistoGene; Laser capture microscopy; Motor neuron; RNA sequencing; Rapid antibody staining; Smart-seq2.

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