Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Oct;13(Suppl 3):S505-S511.
doi: 10.4103/pm.pm_22_17. Epub 2017 Oct 11.

Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia

Ghadah A Alhozaimy  1 , Ebtesam Saad Al-Sheddi  1 , Taghreed A Ibrahim  1   2
Affiliations

Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia

Ghadah A Alhozaimy et al. Pharmacogn Mag. 2017 Oct.

Abstract

Background: Plumeria acutifolia (Apocynaceae) is an ornamental plant, used in the traditional medicine and known to have a variety of constituents as alkaloids, flavonoids, and iridoids. Extracts of this plant were proved to have antimicrobial and anticancer activities.

Objectives: This research was conducted for the evaluation of the biological activities of P. acutifolia stem bark and isolation and structural elucidation of various chemical compounds from the biologically active fractions.

Materials and methods: Methanol extract of stem bark of P. acutifolia was successively fractionated with petroleum ether, dichloromethane, ethyl acetate, and n-butanol. The fractions were evaluated for their antimicrobial, cytotoxic, and antioxidant activities. Fractions with promising biological activities were subjected to chromatographic techniques for the isolation of compounds, followed by structural elucidation using several spectroscopic techniques.

Results: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum (7.81 μg/ml) and Escherichia coli (3.9 μg/ml). The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction, using concentrations of 1, 2.5, 5, and 10 μl/ml. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect, with an IC50 of 197.1 μg/ml. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5).

Conclusion: P. acutifolia was proved to have significant antimicrobial, cytotoxic, and antioxidant activities; the isolated compounds were flavonoids, iridoids, and secoiridoid, some of which were reported for the first time in genus Plumeria and/or family Apocynaceae.

Summary: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum and Escherichia coli. The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). Abbreviations used: mp: Melting point, NMR: Nuclear magnetic resonance, s: Singlet, d: Double, t: Triplet, q: Quartet, dd: Double-double, m: Multiplet, br: Broad.

Keywords: Antimicrobial; Apocynaceae; antioxidant; flavonoids; iridoids.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest

Figures

Figure 1
Figure 1
Cytotoxic activity of stem bark fractions of P. acutifolia against HEPG-2, HCT-116, and MCF-7 cell lines
Figure 2
Figure 2
Percentage of α, α-diphenyl-β-picrylhydrazyl scavenging activity of stem bark fractions of Plumeria acutifolia
Figure 3
Figure 3
COSY and HMBC correlations of compound 4
Figure 4
Figure 4
The structures of compounds 1-5 isolated from stem bark fractions of Plumeria acutifolia
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Endress ME, Bruyns PV. A revised classification of the Apocynceae s.l. Bot Rev. 2000;66:1–56.
    1. Kamariah AS, Lim LB, Baser KH, Ozek T, Demirci B. Composition of the essential oil of Plumeria obtusa L. Flavour Fragr J. 1999;14:237–40.
    1. Perry LM, Metzger J. Medicinal Plants of East and Southest Asia: Attributed Properties and Uses. Cambridge: The MIT Press; 1980. pp. 28–9.
    1. Rasool SN, Jaheerunnisa S, Chitta SK, Jayaveera KN. Antimicrobial activities of Plumeria acutifolia. J Med Plants Res. 2008;2:77–80.
    1. Dobhal MP, Li G, Gryshuk A, Graham A, Bhatanager AK, Khaja SD, et al. Structural modifications of plumieride isolated from Plumeria bicolor and the effect of these modifications on in vitro anticancer activity. J Org Chem. 2004;69:6165–72. - PubMed

LinkOut - more resources