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. 1989 Feb;3(2):174-7.
doi: 10.1096/fasebj.3.2.2914628.

Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration

J C Dunn  1 , M L Yarmush, H G Koebe, R G Tompkins
Affiliations

Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration

J C Dunn et al. FASEB J. 1989 Feb.

Erratum in

  • FASEB J 1989 May;3(7):1873

Abstract

Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.

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