Characterization of three choline transport activities in Rhizobium meliloti: modulation by choline and osmotic stress

Abstract

Choline has both a nutritional and osmoregulatory role in Rhizobium meliloti (T. Bernard, J. A. Pocard, B. Perroud, and D. Le Rudulier, Arch. Microbiol. 143:359-364, 1986). In view of this fact, choline transport was studied in R. meliloti 102F34 to determine how the rate of choline uptake is modulated. The effects of the cultural conditions on the kinetics of transport are presented. A high-affinity activity and a low-affinity activity were found in cells grown in minimal medium. The addition of 0.3 M NaCl or other osmolytes to the medium resulted in a marked decrease in the high-affinity activity, whereas the low-affinity activity remained fairly constant. Furthermore, results from osmotic upshock and downshock experiments indicate that the response of the cell to high osmolarity is rapid; hence, the mechanism of regulation by salt likely does not involve gene induction. A second high-affinity transport activity was induced by choline itself. Like the constitutive low-affinity transport activity, this activity was not greatly altered when the cells were grown in media of elevated osmotic strength. We conclude that although all three kinetically distinct transport systems are efficient at low osmolarity, only the induced high- and low-affinity activities are important for osmoregulation. The characteristics of the three transport activities from R. meliloti are compared with those from other bacterial species that use choline for growth and/or osmoregulation.