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. 1989 Jan;171(1):596-601.
doi: 10.1128/jb.171.1.596-601.1989.

Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp

T Ishizaki  1 , N Hirayama, H Shinkawa, O Nimi, Y Murooka
Affiliations

Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp

T Ishizaki et al. J Bacteriol. 1989 Jan.

Abstract

The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total amino acid composition and amino acid sequencing of the first 21 amino acids from the N terminus of the purified extracellular enzyme agree with the values deduced from nucleotide sequencing data.

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