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. 2017 Nov 17;14(11):1400.
doi: 10.3390/ijerph14111400.

Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

Jeeyeon Lee  1   2 , Jiyeon Jeong  3 , Heeyoung Lee  4 , Jimyeong Ha  5   6 , Sejeong Kim  7   8 , Yukyung Choi  9   10 , Hyemin Oh  11   12 , Kunho Seo  13 , Yohan Yoon  14   15 , Soomin Lee  16
Affiliations

Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

Jeeyeon Lee et al. Int J Environ Res Public Health. .

Abstract

This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in Campylobacter isolates from poultry. Chicken (n = 152) and duck (n = 154) samples were collected from 18 wet markets in Korea. Campylobacter spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLabTM system and were tested for the presence of cytolethal distending toxin (cdt) genes. Campylobacter spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of Campylobacter contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three cdt genes (cdtA, cdtB, and cdtC) were present in 71.1% of Campylobacter isolates. These results indicate that Campylobacter contamination should be decreased to prevent and treat Campylobacter foodborne illness.

Keywords: Campylobacter; Rep-PCR; antibiotic susceptibility; cdt toxin; poultry.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The locations of wet markets for poultry samples collected in Korea.
Figure 2
Figure 2
Dendrogram and gel-like image of the DiversiLab systems for Campylobacter isolates from poultry samples.
See this image and copyright information in PMC

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