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. 2017 Sep 23;8(50):87472-87479.
doi: 10.18632/oncotarget.21267. eCollection 2017 Oct 20.

TIPE2 expression is increased in peripheral blood mononuclear cells from patients with rheumatoid arthritis

Zhu Shi-Bai  1 , Liu Rui-Min  2 , Sun Ying-Chuan  2 , Zhai Jie  3 , Jiang Chao  1 , Ye Can-Hua  1 , Chen Xi  1 , Qian Wen-Wei  1
Affiliations

TIPE2 expression is increased in peripheral blood mononuclear cells from patients with rheumatoid arthritis

Zhu Shi-Bai et al. Oncotarget. .

Abstract

We investigated the changes in mRNA and protein expression of tumor necrosis factor-α-induced protein 8-like 2 (TIPE2) and PEST-containing nuclear protein (PCNP) in peripheral blood lymphocytes from 54 patients with rheumatoid arthritis (RA) and the spleens of model mice with collagen-induced arthritis (CIA) to generate new ideas for clinical diagnosis and treatment. Expression levels of both TIPE2 and PCNP were higher in RA patients and CIA mice than in their respective controls. They were also higher in the 32 patients with active RA than in the 22 with inactive RA (P < 0.001 for both). After comprehensively treating patients with active RA with anti-inflammatory and antirheumatic drugs for 6 months, they were stable, and there was no difference in TIPE2 levels between the treated patients and those with inactive RA (P = 0.85). In addition, TIPE2 mRNA levels in peripheral blood correlated positively with PCNP (R2 = 0.744, P = 0.001). The DAS28 score correlated positively with peripheral blood TIPE2 levels in the RA patients (R2 = 0.945, P = 0.001). These findings suggest TIPE2 expression increases with the severity of RA.

Keywords: PEST-containing nuclear protein (PCNP); rheumatoid arthritis (RA); tumor necrosis factor-α–induced protein 8-like 2 (TIPE2).

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Conflict of interest statement

CONFLICTS OF INTEREST The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1. Histologic analysis in the joints of hind limbs in mice with CIA
(A) Representative joint sections from PBS-treated normal mice stained with hematoxylin and eosin (H&E). (B) Representative joint sections from arthritic mice stained with H&E.
Figure 2
Figure 2. Reverse transcriptase PCR results of TIPE2 and PCNP in spleen cells of CIA mice and normal mice
M is the marker DL2000; 1 to 5 present the quantity of expressed genes in five CIA mice; and 6 and 7 are the quantity of expressed genes in the control groups. Gene expression in CIA mice groups is significantly greater than that in the control groups.
Figure 3
Figure 3. TIPE2 relative expression levels
Activate RA versus inactive RA, *P < 0.001; active RA versus treated RA, #P < 0.001; inactive RA versus treated RA, P = 0.85.
Figure 4
Figure 4. Comparison of the PCNP relative expression levels in patients with inactive and active RA
Active RA and inactive RA versus control group, P < 0.001; active RA versus inactive RA, *P < 0.001.
Figure 5
Figure 5. Western blot results
(A) The left column shows binds of proteins expressed in CIA mice; the right column shows binds of proteins expressed in the control group. Protein expression in CIA mice was significantly greater than that in the control group. (B) There are three groups of human lymphocytes. Protein expression in the RA groups was significantly greater than that in the control group.
Figure 6
Figure 6. Correlation analysis between TIPE2 expression level and DAS28 score in RA patients
Figure 7
Figure 7. Correlation analysis between TIPE2 and PCNP expression in RA patients
See this image and copyright information in PMC

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