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. 2018 Jun 15:199:465-471.
doi: 10.1016/j.saa.2017.11.013. Epub 2017 Nov 6.

Fluorenone based fluorescent probe for selective "turn-on" detection of pyrophosphate and alanine

Daniel Thangadurai T  1 , Nithya I  2 , Manjubaashini N  2 , Bhuvanesh N  3 , Bharathi G  4 , Nandhakumar R  3 , Nataraj D  4
Affiliations

Fluorenone based fluorescent probe for selective "turn-on" detection of pyrophosphate and alanine

Daniel Thangadurai T et al. Spectrochim Acta A Mol Biomol Spectrosc. .

Abstract

To sense biologically important entities with different size and dimensions, a fluorenone based fluorescent receptor was designed and synthesized. Probe 1 displayed a distinct fluorescence enhancement emission at 565nm for pyrophosphate and 530nm for alanine in polar solvent. The fluorescence titration experiments confirm 1:1 stoichiometric ratio with high-binding constant and very low limit of detection (LoD) values. Receptor 1 showed a highly selective and sensitive recognition to HP2O73- and to alanine over other competitive anions and amino acids. In addition, the fluorescence lifetime measurement and reversible binding study results support the practical importance of 1.

Keywords: Biological entities; Fluorenone; H-bonds; Stoichiometry; Zwitterionic form.

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