Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan:66:113-120.
doi: 10.1016/j.ijid.2017.11.019. Epub 2017 Nov 15.

Genotyping and macrolide resistance of Mycoplasma pneumoniae identified in children with community-acquired pneumonia in Medellín, Colombia

Angela Rocio Copete  1 , Yudy Alexandra Aguilar  2 , Zulma Vanessa Rueda  3 , Lázaro Agustín Vélez  4
Affiliations

Genotyping and macrolide resistance of Mycoplasma pneumoniae identified in children with community-acquired pneumonia in Medellín, Colombia

Angela Rocio Copete et al. Int J Infect Dis. 2018 Jan.

Abstract

Objectives: The aim of this study was to describe the genotypes and the main characteristics of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae in hospitalized children in Medellín and neighboring municipalities during the period 2011-2012.

Methods: The M. pneumoniae genotype was determined by PCR and sequencing of the p1 and 23S rRNA genes from induced sputum samples and nasopharyngeal swabs (NPS). Samples were obtained from children with CAP who were hospitalized in 13 healthcare centers. In addition, a spatio-temporal analysis was performed to identify the potential risk areas and clustering of the cases over time.

Results: A variant of type 2 was the dominant genotype in the induced sputum (96.1%) and NPS (89.3%) samples; the type 1 variant was identified in 3.9% and 10.7% of these samples, respectively. No strains with mutations in the 23S rRNA gene associated with macrolide resistance were found. The cases in Medellín were mainly concentrated in the northeastern areas and western districts. However, no temporal relationship was found among these cases.

Conclusions: A variant of type 2 of M. pneumoniae prevailed among children with CAP during the study period. No strains with mutations associated with macrolide resistance were found.

Keywords: CAP; Children; Macrolide-resistant Mycoplasma pneumoniae; Mycoplasma pneumoniae; p1 typing.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Distribution of genotypes in the respiratory samples assessed: genotypes and variants of Mycoplasma pneumoniae identified through typing of the p1 gene in the respiratory samples of children with community-acquired pneumonia collected in Medellín, Bello, Envigado, and Itagui, Colombia, from August 2011 to September 2012.
Figure 2
Figure 2
Spatial distribution of the cases of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae: mapping in Medellín of the spatial distribution of the 48 cases of CAP associated with M. pneumoniae, according to the scale of the Getis-Ord Gi* index. Two risk areas were found in the city based on the scale of colors determined by the index.
Figure 3
Figure 3
Temporal distribution of the cases of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae: the figure shows the distribution of total cases of CAP and those associated with M. pneumoniae over the 14-month recruitment of patients in the macro study. The number on the bars indicates the percentage of CAP cases caused by M. pneumoniae among the total number of cases recruited at that time.
See this image and copyright information in PMC

References

    1. Bébéar Cécile, Pereyre Sabine, Peuchant Olivia. Mycoplasma pneumoniae: susceptibility and resistance to antibiotics. Future Microbiol. 2011;6(4):423–431. doi: 10.2217/fmb.11.18. - DOI - PubMed
    1. Chironna M., Sallustio A., Esposito S., Perulli M., Chinellato I., Di Bari C. Emergence of macrolide-resistant strains during an outbreak of Mycoplasma pneumoniae infections in children. J Antimicrob Chemother. 2011;66(4):734–737. doi: 10.1093/jac/dkr003. - DOI - PubMed
    1. Cho Min-Chul, Kim Hyewon, An Dongheui, Lee Miyoung, Noh Shin-Ae, Kim Mi-Na. Comparison of sputum and nasopharyngeal swab specimens for molecular diagnosis of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila. Ann Lab Med. 2012;32(2):133–138. doi: 10.3343/alm.2012.32.2.133. - DOI - PMC - PubMed
    1. Collier A.M., Clyde W.A. Appearance of Mycoplasma pneumoniae in lungs of experimentally infected hamsters and sputum from patients with natural disease. Am Rev Respir Dis. 1974;110(6):765–773. doi: 10.1164/arrd.1974.110.6P1.765. - DOI - PubMed
    1. Cousin-Allery A., Charron A., de Barbeyrac B., Fremy G., Skov Jensen J., Renaudin H. Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates. Epidemiol Infect. 2000;124(1):103–111. - PMC - PubMed

MeSH terms