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. 2017 Oct 26:15:896-900.
doi: 10.1016/j.dib.2017.10.047. eCollection 2017 Dec.

Draft genome and sequence variant data of the oomycete Pythium insidiosum strain Pi45 from the phylogenetically-distinct Clade-III

Weerayuth Kittichotirat  1 , Preecha Patumcharoenpol  1   2 , Thidarat Rujirawat  3 , Tassanee Lohnoo  3 , Wanta Yingyong  3 , Theerapong Krajaejun  4
Affiliations

Draft genome and sequence variant data of the oomycete Pythium insidiosum strain Pi45 from the phylogenetically-distinct Clade-III

Weerayuth Kittichotirat et al. Data Brief. .

Abstract

Pythium insidiosum is a unique oomycete microorganism, capable of infecting humans and animals. The organism can be phylogenetically categorized into three distinct clades: Clade-I (strains from the Americas); Clade-II (strains from Asia and Australia), and Clade-III (strains from Thailand and the United States). Two draft genomes of the P. insidiosum Clade-I strain CDC-B5653 and Clade-II strain Pi-S are available in the public domain. The genome of P. insidiosum from the distinct Clade-III, which is distantly-related to the other two clades, is lacking. Here, we report the draft genome sequence of the P. insidiosum strain Pi45 (also known as MCC13; isolated from a Thai patient with pythiosis; accession numbers BCFM01000001-BCFM01017277) as a representative strain of the phylogenetically-distinct Clade-III. We also report a genome-scale data set of sequence variants (i.e., SNPs and INDELs) found in P. insidiosum (accessible online at the Mendeley database: http://dx.doi.org/10.17632/r75799jy6c.1).

Keywords: Draft genome; Pythiosis; Pythium insidiosum; Sequence variant.

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Figures

Fig. 1
Fig. 1
Phylogenetic relationship of Pythium insidiosum: the rDNA-based maximum-likelihood phylogenetic tree categorizes 18 strains of Pythium insidiosum into three distinct clades: Clade-I, Clade-II, and Clade-III. Description of each strain of P. insidiosum can be found in Table 1. The arrows indicate the strains [i.e., CDC-B5653 (labeled as Pi10) and Pi-S (labeled as Pi35)] where genome sequences are publically available, while the arrow head indicates the strain Pi45 where genome data is reported here. The rDNA sequence from Pythium granisporangium (accession number: AY151182) is included as an outgroup. Branch support values of greater than 70% are demonstrated at the nodes. Nucleotide substitution per site is shown at the bottom.
See this image and copyright information in PMC

References

    1. Kamoun S. Molecular genetics of pathogenic oomycetes. Eukaryot. Cell. 2003;2:191–199. - PMC - PubMed
    1. Gaastra W., Lipman L.J.A., De Cock A.W.A.M., Exel T.K., Pegge R.B.G., Scheurwater J., Vilela R., Mendoza L. Pythium insidiosum: an overview. Vet. Microbiol. 2010;146:1–16. - PubMed
    1. Krajaejun T., Sathapatayavongs B., Pracharktam R., Nitiyanant P., Leelachaikul P., Wanachiwanawin W., Chaiprasert A., Assanasen P., Saipetch M., Mootsikapun P., Chetchotisakd P., Lekhakula A., Mitarnun W., Kalnauwakul S., Supparatpinyo K., Chaiwarith R., Chiewchanvit S., Tananuvat N., Srisiri S., Suankratay C., Kulwichit W., Wongsaisuwan M., Somkaew S. Clinical and epidemiological analyses of human pythiosis in Thailand. Clin. Infect. Dis. 2006;43:569–576. - PubMed
    1. Krajaejun T., Pracharktam R., Wongwaisayawan S., Rochanawutinon M., Kunakorn M., Kunavisarut S. Ocular pythiosis: is it under-diagnosed? Am. J. Ophthalmol. 2004;137:370–372. - PubMed
    1. Chareonsirisuthigul T., Khositnithikul R., Intaramat A., Inkomlue R., Sriwanichrak K., Piromsontikorn S., Kitiwanwanich S., Lowhnoo T., Yingyong W., Chaiprasert A., Banyong R., Ratanabanangkoon K., Brandhorst T.T., Krajaejun T. Performance comparison of immunodiffusion, enzyme-linked immunosorbent assay, immunochromatography and hemagglutination for serodiagnosis of human pythiosis. Diagn. Microbiol. Infect. Dis. 2013;76:42–45. - PubMed