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. 2018 Jan 2:455:32-38.
doi: 10.1016/j.carres.2017.11.008. Epub 2017 Nov 21.

Microfluidic reactor for lipase-catalyzed regioselective synthesis of neohesperidin ester derivatives and their antimicrobial activity research

Lihua Du  1 , Zhipeng Jiang  2 , Liangliang Xu  2 , Nani Zhou  2 , Jiahong Shen  2 , Zhen Dong  2 , Le Shen  2 , Hong Wang  2 , Xiping Luo  3
Affiliations

Microfluidic reactor for lipase-catalyzed regioselective synthesis of neohesperidin ester derivatives and their antimicrobial activity research

Lihua Du et al. Carbohydr Res. .

Abstract

Lipase-catalyzed regioselective synthesis of neohesperidin ester derivatives was performed by Lipase TL IM from Thermomyces lanuginosus in a continuous-flow microreactor and then their antimicrobial activity was studied. It appears that neohesperidin, neohesperidin dihydrochalcone with primary OH on the sugar part is the most reactive substrate. Various reaction parameters were investigated including substrate molar ratio, reaction time and temperature. Maximum conversion (92%) was obtained under the optimal condition of substrate molar ratio of 8:1 (vinyl esters: neohesperidin) at 52 °C for about 35 min. Then, the antibacterial activity of modified neohesperidin ester derivatives was examined and showed great improvement against gram negative and gram positive bacteria.

Keywords: Enzymatic synthesis; Lipases; Microreactor; Neohesperidin; Regioselectivity.

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