Transgenic Peanut (Arachis hypogaea L.) Overexpressing mtlD Gene Showed Improved Photosynthetic, Physio-Biochemical, and Yield-Parameters under Soil-Moisture Deficit Stress in Lysimeter System

Abstract

Peanut, an important oilseed crop, frequently encounters drought stress (DS) during its life cycle. In this study, four previously developed mtlD transgenic (T) peanut lines were used for detailed characterization under DS, at the reproductive stage using lysimeter system under controlled greenhouse conditions. In dry-down experiments, T lines maintained better photosynthetic machinery, such as, photosynthesis rate, stomatal conductance, transpiration rate, and SPAD (Soil-Plant Analyses Development) values, and had lower oxidative damage, including lipid membrane peroxidation and hydrogen peroxide and superoxide radical accumulation than WT, when exposed to 24 days of DS. WT plants had a more negative water potential (WP; up to -3.22 MPa) than T lines did (-2.56 to -2.71 MPa) at day 24 of DS treatment. During recovery, T lines recovered easily whereas 67% of WT plants failed to recover. In T lines, the rate of photosynthesis strongly and positively correlated with the transpiration rate (r = 0.92), RWC (r = 0.90), WP (r = 0.86), and total chlorophyll content (r = 0.75), suggesting its strong correlation with water retention-related parameters. Furthermore, yield parameters such as, pod weight and harvest index of T lines were up to 2.19 and 1.38 times more than those of WT plants, respectively. Thus, the significantly better performance of mtlD T peanut lines than of WT plants under DS could be attributed to the accumulation of mannitol, which in turn helped in maintaining the osmoregulation and ROS scavenging activity of mannitol and ultimately conferred water-economizing capacity and higher yield in T lines than in WT plants.

Keywords: drought stress; growth-related traits; lysimeter system; peanut; physio-biochemical parameters; wilting symptoms.

Figure 1

Estimation of photosynthetic rate (A), stomatal conductance (B), transpiration rate (C), and SPAD values (D) of wild-type and transgenic lines at 0, 10, and 24 days under drought-stress conditions. The mean values ±SE (n = 3) followed by similar lower case letters within a column are not significantly different (P ≤ 0.05).

Figure 2

In situ localization of hydrogen peroxide (A) and the detection of superoxide radicals (B) and cell-death (C) in wild-type and transgenic lines under well-watered and drought stressed condition using DAB, NBT, and trypan blue staining, respectively.

Figure 3

Comparison of soil-moisture content at 30 and 45 cm depths at 0, 10, and 24 days under well-watered and drought-stress conditions in wild-type and transgenic lines. Where, error bars on the line are SEM, while those on top represent LSD_(0.05).

Figure 4

Comparisons of phenotypic traits between wild-type and transgenic lines at 24 days under well-watered and drought-stressed conditions.

Figure 5

Variations in root growth between wild-type and transgenic lines under well-watered and drought-stressed condition after harvesting.