Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Mar;44(3):388-96.

Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction

J L Weber  1 , P E May
Affiliations

Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction

J L Weber et al. Am J Hum Genet. 1989 Mar.

Abstract

Interspersed DNA elements of the form (dC-dA)n.(dG-dT)n constitute one of the most abundant human repetitive DNA families. We report that specific human (dC-dA)n.(dG-dT)n blocks are polymorphic in length among individuals and therefore represent a vast new pool of potential genetic markers. Comparison of sequences from the literature for (dC-dA)n.(dG-dT)n blocks cloned two or more times revealed length polymorphisms in seven of eight cases. Variations in the lengths of 10 (dC-dA)n.(dG-dT)n blocks were directly demonstrated by amplifying the DNA within and immediately flanking the repeat blocks by using the polymerase chain reaction and then resolving the amplified DNA on polyacrylamide DNA sequencing gels. Use of the polymerase chain reaction to detect DNA polymorphisms offers improved sensitivity and speed compared with standard blotting and hybridization.

PubMed Disclaimer

References

    1. Exp Parasitol. 1988 Aug;66(2):143-70 - PubMed
    1. Nucleic Acids Res. 1988 Feb 11;16(3):865-81 - PubMed
    1. Genomics. 1988 May;2(4):273-9 - PubMed
    1. EMBO J. 1987 Jun;6(6):1781-9 - PubMed
    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed

Publication types