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Review

Inhibition of Protein-Protein Interactions: Cell-Based Assays

Mark Wade  1 , Jacqui Méndez  2 , Nathan P. Coussens  3 , Michelle R. Arkin  4 , Marcie A. Glicksman  5
Sarine Markossian  1 , Abigail Grossman  1 , Hannah Baskir  1 , Michelle Arkin  2 , Douglas Auld  3 , Christopher Austin  4 , Jonathan Baell  5 , Kyle Brimacombe  1 , Thomas D.Y. Chung  6 , Nathan P. Coussens  7 , Jayme L. Dahlin  8 , Viswanath Devanarayan  9 , Timothy L. Foley  10 , Marcie Glicksman  11 , Kirill Gorshkov  12 , Stefan Grotegut  13 , Matthew D. Hall  1 , Samuel Hoare  14 , James Inglese  1 , Philip W. Iversen  15 , Madhu Lal-Nag  16 , Zhuyin Li  12 , Jason R. Manro  13 , James McGee  17 , Allison Norvil  13 , Mackenzie Pearson  13 , Terry Riss  18 , Peter Saradjian  19 , G. Sitta Sittampalam  20 , Michael A. Tarselli  21 , O. Joseph Trask Jr.  22 , Jeffrey R. Weidner  23 , Mary Jo Wildey  24 , Kelli Wilson  1 , Menghang Xia  1 , Xin Xu  1
, editors.
In: Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
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Review

Inhibition of Protein-Protein Interactions: Cell-Based Assays

Mark Wade et al.
Free Books & Documents

Excerpt

There is a strong interest in discovering compounds that inhibit protein-protein interactions. High-throughput screening (HTS) approaches include formats using purified proteins (see AGM chapter Inhibition of Protein-Protein Interactions: Non-Cellular Assay Formats) (1) and those using whole cells. This chapter describes two types of cell-based HTS assays, energy transfer (Förster resonance energy transfer and bioluminescence resonance energy transfer) and protein complementation (fluorescence or enzymatic, e.g. luciferase).

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References

    1. Arkin MR, Glicksman MA, Fu H, Havel JJ, Du Y. Inhibition of Protein-Protein Interactions: Non-Cellular Assay Formats. In: Sittampalam GS, Coussens NP, Brimacombe K, Grossman A, Arkin M, Auld D, Austin C, Baell J, Bejcek B, Chung TDY, Dahlin JL, Devanaryan V, Foley TL, Glicksman M, Hall MD, Hass JV, Inglese J, Iversen PW, Kahl SD, Kales SC, Lal-Nag M, Li Z, McGee J, McManus O, Riss T, Trask OJ, Jr., Weidner JR, Xia M, Xu X, editors. Assay Guidance Manual. Bethesda (MD)2004.
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