Phorbol ester-induced activation of protein kinase C leads to increased formation of diacylglycerol in human neutrophils
- PMID: 2917604
- DOI: 10.1016/0014-4827(89)90195-x
Phorbol ester-induced activation of protein kinase C leads to increased formation of diacylglycerol in human neutrophils
Abstract
Human neutrophils stimulated with a phorbol ester (phorbol 12-myristrate 13-acetate or phorbol 12,13-dibutyrate) responded with an increase in diacylglycerol, considered the natural activator of protein kinase C. The amounts of diacylglycerol formed were considerable, reaching 700-900% of basal after 20 min. In contrast, 4-alpha-phorbol 12-myristate 13-acetate did not induce any detectable formation of diacylglycerol. Simultaneously, phorbol 12-myristate 13-acetate exposure caused increased breakdown of both phosphatidylcholine and phosphatidylinositol 4,5-bisphosphate. These results suggest that once activated, protein kinase C can positively modulate its own activity by inducing additional formation of diacylglycerol from at least two different sources.
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