Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

Kenneth W Witwer¹, Carolina Soekmadji², Andrew F Hill³, Marca H Wauben⁴, Edit I Buzás⁵, Dolores Di Vizio⁶, Juan M Falcon-Perez^{7

8}, Chris Gardiner⁹, Fred Hochberg^{10

11}, Igor V Kurochkin¹², Jan Lötvall^{13

14}, Suresh Mathivanan³, Rienk Nieuwland¹⁵, Susmita Sahoo¹⁶, Hidetoshi Tahara¹⁷, Ana Claudia Torrecilhas¹⁸, Alissa M Weaver¹⁹, Hang Yin^{20

21}, Lei Zheng²², Yong Song Gho²³, Peter Quesenberry²⁴, Clotilde Théry²⁵

Affiliations

¹ Departments of Molecular and Comparative Pathobiology and Neurology, The Johns Hopkins University School of Medicine, Baltimore, USAkwitwer1@jhmi.edu.
² Department of Cell and Molecular Biology, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
³ Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia.
⁴ Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
⁵ Department of Genetics, Cell- and Immunobiology, Semmelweis University and MTA-SE Immunoproteogenomics Extracellular Vesicle Research Group, Budapest, Hungary.
⁶ Division of Cancer Biology and Therapeutics, Departments of Surgery, Biomedical Sciences, and Pathology and Laboratory Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
⁷ Exosomes Laboratory & Metabolomics Platform, CIC bioGUNE, CIBERehd, Derio, Spain.
⁸ IKERBASQUE Research Science Foundation, Bilbao, Spain.
⁹ Research Department of Haematology, University College London, London, UK.
¹⁰ Neurosurgery, University of California at San Diego, San Diego, CA, USA.
¹¹ The Scintillon Institute, La Jolla, CA, USA.
¹² Central Research Laboratories, Sysmex Co, Kobe, Japan.
¹³ Codiak BioSciences, Cambridge, MA, USA.
¹⁴ Krefting Research Centre, University of Gothenburg, Gothenburg, Sweden.
¹⁵ Department of Clinical Chemistry and Vesicle Observation Centre, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
¹⁶ Department of Medicine, Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
¹⁷ Department of Cellular and Molecular Biology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
¹⁸ Departamento de Ciências Farmacêuticas, UNIFESP, Diadema, Brazil.
¹⁹ Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
²⁰ Center of Basic Molecular Science, Department of Chemistry, Tsinghua University, Beijing, China.
²¹ Department of Chemistry and Biochemistry and the BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, USA.
²² Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
²³ Department of Life Sciences, POSTECH (Pohang University of Science and Technology), Pohang, South Korea.
²⁴ Brown Medical School, Rhode Island Hospital, Providence, RI, USA.
²⁵ Institut Curie, INSERM U932, PSL Research University, Paris, Franceclotilde.thery@curie.fr.

PMID: 29184626
PMCID: PMC5698937
DOI: 10.1080/20013078.2017.1396823

Editorial

Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

Kenneth W Witwer et al. J Extracell Vesicles. 2017.

. 2017 Nov 15;6(1):1396823.

doi: 10.1080/20013078.2017.1396823. eCollection 2017.

Authors

Affiliations

¹ Departments of Molecular and Comparative Pathobiology and Neurology, The Johns Hopkins University School of Medicine, Baltimore, USAkwitwer1@jhmi.edu.
² Department of Cell and Molecular Biology, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
³ Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia.
⁴ Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
⁵ Department of Genetics, Cell- and Immunobiology, Semmelweis University and MTA-SE Immunoproteogenomics Extracellular Vesicle Research Group, Budapest, Hungary.
⁶ Division of Cancer Biology and Therapeutics, Departments of Surgery, Biomedical Sciences, and Pathology and Laboratory Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
⁷ Exosomes Laboratory & Metabolomics Platform, CIC bioGUNE, CIBERehd, Derio, Spain.
⁸ IKERBASQUE Research Science Foundation, Bilbao, Spain.
⁹ Research Department of Haematology, University College London, London, UK.
¹⁰ Neurosurgery, University of California at San Diego, San Diego, CA, USA.
¹¹ The Scintillon Institute, La Jolla, CA, USA.
¹² Central Research Laboratories, Sysmex Co, Kobe, Japan.
¹³ Codiak BioSciences, Cambridge, MA, USA.
¹⁴ Krefting Research Centre, University of Gothenburg, Gothenburg, Sweden.
¹⁵ Department of Clinical Chemistry and Vesicle Observation Centre, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
¹⁶ Department of Medicine, Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
¹⁷ Department of Cellular and Molecular Biology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
¹⁸ Departamento de Ciências Farmacêuticas, UNIFESP, Diadema, Brazil.
¹⁹ Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
²⁰ Center of Basic Molecular Science, Department of Chemistry, Tsinghua University, Beijing, China.
²¹ Department of Chemistry and Biochemistry and the BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, USA.
²² Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
²³ Department of Life Sciences, POSTECH (Pohang University of Science and Technology), Pohang, South Korea.
²⁴ Brown Medical School, Rhode Island Hospital, Providence, RI, USA.
²⁵ Institut Curie, INSERM U932, PSL Research University, Paris, Franceclotilde.thery@curie.fr.

PMID: 29184626
PMCID: PMC5698937
DOI: 10.1080/20013078.2017.1396823

No abstract available

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

**Figure 1.**
Community engagement with MISEV.

**Figure 2.**
Opinions about minimal requirements in the EV field.

**Figure 3.**
Who should establish and update minimal requirements?

**Figure 4.**
MISEV: suggested retentions and additions.

See this image and copyright information in PMC

References

1. Lotvall J, Hill AF, Hochberg F, et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles [Internet]. 2014;3:26913 Available from http://www.ncbi.nlm.nih.gov/pubmed/25536934 - PMC - PubMed
1. Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem [Internet]. 2009. February 28;55(4):611–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19246619 - PubMed
1. Brazma A, Hingamp P, Quackenbush J, et al. Minimum information about a microarray experiment (MIAME)-toward standards for microarray data. Nat Genet [Internet]. 2001. December 01;29(4):365–371. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11726920 - PubMed
1. Lee JA, Spidlen J, Boyce K, et al. MIFlowCyt: the minimum information about a flow cytometry experiment. Cytom Part A [Internet]. 2008. October;73A(10):926–930. [cited 2017 Sep 16]. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18752282 - PMC - PubMed
1. van Deun J, Hendrix A.. Is your article EV-TRACKed? J Extracell Vesicles. Forthcoming 2017;1. - PMC - PubMed

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Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

Affiliations

Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

Authors

Affiliations

Conflict of interest statement

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