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Comment
. 2017 Nov 29;49(12):1674-1675.
doi: 10.1038/ng.3994.

Correcting CRISPR for copy number

John Paul Shen  1 , Trey Ideker  1
Affiliations
Comment

Correcting CRISPR for copy number

John Paul Shen et al. Nat Genet. .

Abstract

The CRISPR-Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

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References

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    1. Nat Methods. 2015 Sep;12(9):823-6 - PubMed
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