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. 2017 Nov 30;8(12):346.
doi: 10.3390/genes8120346.

Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01

Guoqiang Zhang  1   2 , Dailin Yu  3 , Bu Sang  4 , Juntao Feng  5 , Lirong Han  6 , Xing Zhang  7
Affiliations

Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01

Guoqiang Zhang et al. Genes (Basel). .

Abstract

Streptomyces kanasensis ZX01 produces some antibiotics and a glycoprotein with antiviral activity. To further evaluate its biosynthetic potential, here we sequenced the 7,026,279 bp draft genome of S. kanasensis ZX01 and analyzed all identifiable secondary gene clusters for controlling natural products. More than 60 putative clusters were found in S. kanasensis ZX01, the majority of these biosynthetic loci are novel. In addition, the regulators for secondary metabolism in S. kanasensis ZX01 were abundant. The global regulator nsdA not only controls biosynthesis of some antibiotics, but also enhances production of glycoprotein GP-1 with antiviral activity. This study importantly reveals the powerful interplay between genomic analysis and studies of traditional natural product purification/production increasing.

Keywords: genome mining; glycoprotein GP-1; nsdA; regulator; secondary metabolism.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Proposed PKS/NRPs module and domain organization in five clusters. PKS: polyketide biosynthase, NRPs: nonribosomal peptide synthetase, KS: ketosynthase, AT: acyltransferase, DH: dehydratase, ER: enoyl reductase, KR: ketoreductase, ACP: acyl carrier protein, cMT: C-methyl transferase, PCP: peptidyl carrier protein, C: condensation, A: adenylation, PDN: PKS docking N-term, PDC: PKS docking C-term, NCN: NRPS-COM N-term, TE: thioesterase.
Figure 2
Figure 2
Expression difference of PKS and NRPs genes in S. kanasensis ZX01 after γ-butyrolactone induction for 72 h. Ska0065, Ska1968 and Ska3482 are type-I PKS; Ska4292 is type-III PKS; Ska3506 and Ska5321 is NRPs.
Figure 3
Figure 3
Differences between wild type and mutant of S. kanasensis ZX01 in morphology and production of glycoprotein GP-1. (A) wild type strain of S. kanasensis ZX01; (B) nsdA disruption mutant in which nsdA was replaced by kanamycin resistance gene (kan); (1) Two strains were cultivated on the Gause-I plate medium for 5 days; (2) Two strains was cultivated in GCBY liquid medium for 3 days; (3) The differences of biomass between wild type and ΔnsdA mutant; (4) Determination of glycoprotein GP-1 in wild type and ΔnsdA mutant.
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