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. 2018:1695:97-107.
doi: 10.1007/978-1-4939-7407-8_10.

Shotgun Sphingolipid Analysis of Human Aqueous Humor

Anna Trzeciecka  1 , Ulises Arbelo  2 , Arturo Barron  2 , Genea Edwards  2 , Sruthi Sampathkumar  3 , Carol Toris  3 , Sanjoy K Bhattacharya  4
Affiliations

Shotgun Sphingolipid Analysis of Human Aqueous Humor

Anna Trzeciecka et al. Methods Mol Biol. 2018.

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Keywords: Aqueous humor; Ceramide; Electrospray ionization-tandem mass spectrometry (ESI-MS/MS); MZmine 2.21; Shotgun lipidomics; Sphingolipids; Sphingomyelin; Sphingosine; Sphingosine-1-phosphate.

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