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. 2017 Sep 28;8(54):92420-92430.
doi: 10.18632/oncotarget.21367. eCollection 2017 Nov 3.

Dietary apple pectic oligosaccharide improves gut barrier function of rotavirus-challenged weaned pigs by increasing antioxidant capacity of enterocytes

Xiangbing Mao  1   2 , Xiangjun Xiao  1   2 , Daiwen Chen  1   2 , Bing Yu  1   2 , Jun He  1   2 , Hao Chen  1   2 , Xuechun Xiao  1   2 , Junqiu Luo  1   2 , Yuheng Luo  1   2 , Gang Tian  1   2 , Jianping Wang  1   2
Affiliations

Dietary apple pectic oligosaccharide improves gut barrier function of rotavirus-challenged weaned pigs by increasing antioxidant capacity of enterocytes

Xiangbing Mao et al. Oncotarget. .

Abstract

Rotavirus can lead to decreasing gut barrier function and diarrhea of children and young animals. Apple pectic oligosaccharide treatment reduced diarrhea in rotavirus-infected piglets. This study was conducted to explore whether apple pectic oligosaccharide administration could protect gut barrier function of piglets against rotavirus infection. A total of 28 crossbred weaned barrows were allotted into 2 treatments fed the diets supplementing 0 and 200 mg/kg apple pectic oligosaccharide. Half of pigs in each diet treatment were challenged by rotavirus on d 15. The whole duration of this experiment is 18 days. Rotavirus challenge increased average diarrhea index, and impaired microbiota in cecal digesta, and histology, expressions of tight-junction proteins, mucins and glucagon like peptide-2 concentrations, antioxidant capacity, endoplasmic reticulum stress, autophagy and apoptosis in jejunal mucosa of piglets. However, dietary apple pectic oligosaccharide supplementation relieved effects of rotavirus challenge on diarrhea, gut health, and antioxidant capacity, endoplasmic reticulum stress, autophagy and apoptosis of jejunal mucosa in piglets. These results suggest that apple pectic oligosaccharide administration can prevent diarrhea and damage of gut barrier function via improving antioxidant capacity that might reduce endoplasmic reticulum stress, autophagy and apoptosis of intestinal epithelial cells in rotavirus-infected piglets.

Keywords: antioxidant capacity; apple pectic oligosaccharide; gut barrier function; rotavirus; weaned piglets.

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Conflict of interest statement

CONFLICTS OF INTEREST The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1. The jejunal mucosal morphology of the weaned pigs
(A) The pig fed the basal diet and orally infused with the sterile essential medium; (B) the pig fed the APOS supplementing diet and orally infused with the sterile essential medium; (C) the pig fed the basal diet and orally infused with rotavirus; (D) the pig fed the APOS supplementing diet and orally infused with rotavirus. (Original magnification, 100 ×).
Figure 2
Figure 2. The effect of dietary APOS supplementation and/or RV challenge on the levels of ZO-1, occludin, claudin 1 and claudin 3 in the jejunal mucosa of weaned pigs
Representative Western blots for ZO-1, occludin, claudin 1, claudin 3 and β-actin in the jejunal mucosa of weaned pigs were shown. Results were expressed as the amount of ZO-1, occludin, claudin 1 and claudin 3 to β-actin in each treatment as a ratio of the other pigs to the unchallenging pigs fed basal diet. Values are means ± SE; n = 7. Values with different letters are significantly different (P < 0.05).
Figure 3
Figure 3. The effect of dietary APOS supplementation and/or RV challenge on the levels of p-mTOR, mTOR, Beclin 1, CHOP, Bax and Bcl-2 in the jejunal mucosa of weaned pigs
Representative Western blots for p-mTOR, mTOR, Beclin 1, CHOP, Bax, Bcl-2 and β-actin in the jejunal mucosa of weaned pigs were shown. Results were expressed as the amount of p-mTOR, mTOR, Beclin 1, CHOP, Bax and Bcl-2 to β-actin in each treatment as a ratio of the other pigs to the unchallenging pigs fed basal diet. Values are means ± SE; n = 7. Values with different letters are significantly different (P < 0.05).
See this image and copyright information in PMC

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