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. 1979 Oct;76(10):5000-4.
doi: 10.1073/pnas.76.10.5000.

Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength

A Stein, J P Whitlock Jr, M Bina

Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength

A Stein et al. Proc Natl Acad Sci U S A. 1979 Oct.

Abstract

We provide evidence that nucleosomes can assemble in vitro at physiological ionic strength (0.1-0.2 M NaCl/10 mM Tris . HCl, pH 8.0) in the absence of "assembly factors" and that poly(glutamic acid) greatly facilitates chromatin assembly under these conditions. We also show that in the presence of either poly(glutamic acid) or poly(aspartic acid), core histones assemble into octamers at physiological ionic strength. We suggest that it is a property of histones to assemble into octamers upon their interaction with macromolecules containing regions of high negative charge density, and we discuss several implications of this property.

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References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:287-300 - PubMed
    1. Cell. 1977 Jul;11(3):609-18 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2780-4 - PubMed

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