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. 2017 Dec 1;2017(12):pdb.prot098640.
doi: 10.1101/pdb.prot098640.

Immunoprecipitation

James DeCaprio, Thomas O Kohl

Immunoprecipitation

James DeCaprio et al. Cold Spring Harb Protoc. .

Abstract

This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the enzyme-linked immunosorbent assay (ELISA), western blotting, or mass spectrometry.

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