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. 1979 Sep;76(9):4503-6.
doi: 10.1073/pnas.76.9.4503.

Biological detection of specific mRNA molecules by microinjection

C P Liu, D L Slate, R Gravel, F H Ruddle

Biological detection of specific mRNA molecules by microinjection

C P Liu et al. Proc Natl Acad Sci U S A. 1979 Sep.

Abstract

We have used direct microinjection of messenger RNA into individual mouse and human cells to assay for specific translation products. We have been able to detect the synthesis of human fibroblast interferon, thymidine, kinase, hypoxanthine phosphoribosyltransferase, adenine phosphoribosyltransferase, and propionyl-CoA carboxylase in response to injected mRNA. Using the interferon system as a model, we have quantitated interferon synthesis and followed partial purification of interferon mRNA sequences on sucrose density gradients. The methods we have utilized should be applicable to other systems in which sensitive assays exist for gene products and should provide a screening procedure for isolating specific mRNA sequences.

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References

    1. Cell. 1976 Dec;9(4 PT 2):725-32 - PubMed
    1. Nature. 1977 Sep 29;269(5627):422-5 - PubMed
    1. Cell. 1979 Jan;16(1):171-80 - PubMed
    1. J Gen Virol. 1978 Nov;41(2):325-31 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502-6 - PubMed

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