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. 2017 Summer;16(3):880-892.

Docking Studies, Synthesis, and In-vitro Evaluation of Novel Oximes Based on Nitrones as Reactivators of Inhibited Acetylcholinesterase

Seyed Ayoub Hosseini  1 , Abolghasem Moghimi  1 , Maryam Iman  2   3 , Firoz Ebrahimi  4
Affiliations

Docking Studies, Synthesis, and In-vitro Evaluation of Novel Oximes Based on Nitrones as Reactivators of Inhibited Acetylcholinesterase

Seyed Ayoub Hosseini et al. Iran J Pharm Res. 2017 Summer.

Abstract

Acetylcholinesterase has important role in synaptic cleft. It breaks down the acetylcholineat cholinergic synapsesand terminates the cholinergic effects. Some chemical agents like organophosphorus compounds (OPCs) including nerve agents and pesticides react with acetylcholinesteraseirreversibly. They inhibit normal biological enzyme action and result in accumulation of acetylcholineand show toxic effects andcholinergic symptoms. The process of Acetylcholinesterase (AChE) inhibition can be reversed by a nucleophilic agent to dephosphorylate and reactivate the enzyme. In this study, design and docking studies of 15 novel nitrone based onoximes as reactivators were performed by using AutoDock program. Then, more effective reactivatorsoximes in terms of binding energy and orientation within the active site were synthesized and evaluated in-vitro on human AChE (hAChE) inhibited by paraoxon and compared to standard hAChE reactivators (2-PAM and obidoxime). Our results used to design new derivatives of Oxim with better efficacy than 2-PAM and obidoxime. Syntheses of some selected bis-pyridiniumoximes based on the nitrones are underway.

Keywords: Molecular docking; Nitrones; Organophosphorus compounds; Oximes; Reactivators.

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Figures

Figure 1
Figure 1
Commercially available AChE reactivators
Figure 2.
Figure 2.
Docked structures of obidoxime (top) and 2PAM (Bottom) with the crystal structure of tabun-inhibited AChE (PDB code 2JF0). Hydrogen bonds and pi-pi interactions have been represented
Figure 3
Figure 3
Docked structures of 4A (top) and 4B (Bottom) with Crystal structure of tabun-inhibited AChE (PDB code 2JF0). Hydrogen bonds and pi-pi interactions have been represented
Figure 4
Figure 4
Docked structures of 4C (top) and 5B (Bottom) with Crystal structure of tabun-inhibited AChE (PDB code 2JF0). Hydrogen bonds and pi-pi interactions have been represented
Scheme 1
Scheme 1
Synthetic scheme for the novel mono-pyridiniumoximes
See this image and copyright information in PMC

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