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. 2017 Sep;9(3):117-122.
doi: 10.1556/1646.9.2017.17.

Endothelial nitric oxide synthase gene polymorphisms in patients with slow coronary flow

Nurzen Sezgin  1 , Abdullah Tekin  2 , Fatma Belgin Atac  3 , Hasibe Verdi  3 , Alpay Turan Sezgin  4
Affiliations

Endothelial nitric oxide synthase gene polymorphisms in patients with slow coronary flow

Nurzen Sezgin et al. Interv Med Appl Sci. 2017 Sep.

Abstract

Background and aims: The aim of this study was to explore potential associations of the intron 4 variable number of tandem repeats (VNTR) and E298A polymorphisms of the endothelial nitric oxide synthase (eNOS) gene with slow coronary flow (SCF). The association between plasma nitrate and nitrite (NO x ) concentrations and eNOS gene polymorphisms was also assessed.

Materials and methods: The intron 4 VNTR and E298A polymorphisms of the eNOS gene were evaluated in the isolated DNA blood samples obtained from the SCF patient group (n = 30) and healthy group consisted of age- and sex-matched controls (n = 61).

Results: Plasma NO x level was significantly lower in patients with SCF than in controls. In addition, patients with SCF have significantly lower nitric oxide levels than control subjects within each genotype variants. The allele and genotyped frequencies of the eNOS intron 4 VNTR and E298A polymorphisms were similar between patients with SCF and the controls. Plasma NO x concentrations with respect to the relevant genotypes were found insignificant.

Discussion and conclusion: Plasma NO x is lower in patients with SCF than in healthy subjects. Our findings may suggest the lack of association between intron 4 VNTR and E298A polymorphisms of the eNOS gene and SCF.

Keywords: coronary disease; endothelial function; endothelial nitric oxide synthase gene polymorphism; nitric oxide; slow coronary flow.

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Figures

Fig. 1.
Fig. 1.
The differences between the genotypes of eNOS polymorphism (intron 4 VNTR) with respect to plasma NOx levels
Fig. 2.
Fig. 2.
The differences between the genotypes of eNOS polymorphism (exon 7 E298A) with respect to plasma NOx levels
See this image and copyright information in PMC

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