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. 2017 Dec;43(4):434-438.
doi: 10.5152/tud.2017.23356. Epub 2017 Dec 1.

Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Selahattin Çalışkan  1 , Muzaffer Oğuz Keleş  2 , Metin İshak Öztürk  2 , Musab Ali Kutluhan  2 , Olgu Enis Tok  3 , Feriha Ercan  3 , Muhammet İhsan Karaman  2
Affiliations

Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Selahattin Çalışkan et al. Turk J Urol. 2017 Dec.

Abstract

Objective: Efficacy of treatments for benign prostate hyperplasia (BPH) is limited because the disease has complex etiopathogenesis. Recent studies have demonstrated the presence of phosphodiesterase-5 (PDE-5) receptors in prostate tissue. We investigated efficacy of sildenafil citrate in testosteron - induced BPH in rats.

Material and methods: The rats were divided into three groups. Each groups had 7 rats. Group 1 was control group. Testosteron propionate 3 mg/kg/day was injected subcutaneously for two weeks in Group 2. The same procedure was done for Group 3 and sildenafil citrate was added to water at daily doses of 2 mg/kg for two weeks. The rats were euthanized with intraperitoneal pentobarbital. The body weights were measured and the prostates were removed.

Results: The mean weights of rats were 288±31.93, 345±23.23 and 294±32.86 g in Groups 1, 2 and 3, respectively. The mean prostate weights of rats were 0.74±0.18, 1.3±0.13 and 0.72±0.24 g in Groups 1, 2, and 3, respectively. Group 2 had statistically significantly higher prostate weights than the other groups (p<0.01). Relative prostate weight is calculated with ratio of prostate weight to body weight. BPH group showed an increase in relative prostate weight compared with other groups with significant difference (p=0.036 and p=0.040). There was statistical difference for acinar area between Group 2 and the others, no significant difference of number of acini, interstitial space and epithelial thickness. Group 2 has more papillary projections per acini than the other groups.

Conclusion: Favourable effect of sildenafil citrate on dimensions of prostate but not all on histological parameters was observed. We expect that PDE-5 inhibitors might be a treatment option for BPH patients if the studies support our findings in the future.

Keywords: Hyperplasia; prostate; sildenafil citrate.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
Normal acini was seen in Group 1, ×40 magnification with H&E
Figure 2
Figure 2
Multiple papillary projections in acini (*) and thickened epithelium (arrow) in Group 2, ×100 magnification with H&E
Figure 3
Figure 3
Fewer number of papillary projections (*) and normal epithelial height in Group 3 (arrow) ×40 magnification with H&E
See this image and copyright information in PMC

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References

    1. Delella FK, Felisbino SL. Doxazosin Treatment Alters Stromal Cell Behavior and Increases Elastic System Fibers Deposition in Rat Prostate. Microsc Res Tech. 2010;73:1036–44. https://doi.org/10.1002/jemt.20828. - DOI - PubMed
    1. Ejike CECC, Ezeanyika LUS. Inhibition of the Experimental Induction of Benign Prostate Hyperplasia: A Possible Role for Fluted Pumpkin(Telfairia occidentalis Hook f.) Seeds. Urol Int. 2011;87:218–24. - PubMed
    1. Shin IS, Lee MY, Jung DY, Seo CS, Ha HK, Shin HK. Ursolic acid reduces prostate size and dihydrotestosterone level in a rat model of benign prostate hyperplasia. Food Chem Toxicol. 2012;50:884–8. https://doi.org/10.1016/j.fct.2012.01.007. - DOI - PubMed
    1. Sarbishegi M, Khajavi O, Arab MR. Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia. Nephrourol Mon. 2016;5:e39284. - PMC - PubMed
    1. Lee JG, Moon DG, Kang SH, Cho DY, Park HS, Bae JH. Relaxation Effect of Phosphodiesterase-5 Inhibitor on the Animal Bladder and Prostatic Urethra: in vitro and in vivo Study. Urol Int. 2010;84:231–5. https://doi.org/10.1159/000277604. - DOI - PubMed

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