Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:5796456.
doi: 10.1155/2017/5796456. Epub 2017 Oct 19.

Effect of a Carbohydrate-Rich Diet on Rat Detrusor Smooth Muscle Contractility: An Experimental Study

Mustafa Suat Bolat  1 , Sırrı S Bilge  2 , Ekrem Akdeniz  1 , Onder Cinar  1 , Fatih Firat  3 , Arzu Erdal Agri  2 , Mustafa Bakirtas  4 , Omer Alici  5 , Fikret Erdemir  6
Affiliations

Effect of a Carbohydrate-Rich Diet on Rat Detrusor Smooth Muscle Contractility: An Experimental Study

Mustafa Suat Bolat et al. Biomed Res Int. 2017.

Abstract

Objectives: We aimed to investigate the effect of a carbohydrate-rich diet on detrusor contractility in rats.

Materials and methods: Sprague-Dawley rats were randomized into two groups. The control group received regular food and water. The study group received carbohydrate-rich diet for six weeks. The rats' detrusor muscle was isolated for pharmacological and histopathological examinations.

Results: In the control and study groups, mean body weights were 431.5 ± 27.6 g and 528.0 ± 36.2 g, respectively (p < 0.001). Electrical stimulation of the detrusor strips of the control group resulted in gradual contraction. A decreased contractile response was shown in the study group. Acetylcholine in 10-7-10-3 molar concentration produced a decreased contractile response in the study group, compared to the control group (p < 0.01). The study group showed marked subepithelial and intermuscular fibrosis in the bladder.

Conclusion: Carbohydrate-rich diet causes marked subepithelial and extracellular fibrosis and changes in contractility in the detrusor within a six-week period. Changes have higher costs in therapeutic choices and correction of these changes remains difficult. Putting an end to carbohydrate-rich diet would seem to be more cost-effective than dealing with the effects of consuming it in high proportions which should be the national policy worldwide.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effects of a fructose-rich diet on electrical field stimulation-evoked contractions; ∗p < 0.05 and ∗∗p < 0.01, compared to the corresponding frequency of control group.
Figure 2
Figure 2
Recordings of responses of electrical field stimulation evoked (100 V, 0.5 ms, 2–64 Hz) in control and study groups.
Figure 3
Figure 3
(a, c) Histopathological appearance of the urinary bladder of the groups (H&E stain, 100x). (b, d) Masson trichrome stain in both groups, respectively. L: lumen; SE: subepithelium; D: detrusor; S: serosa. Care must be taken with marked subepithelial and extracellular fibrosis (d) when compared to the control group (b).
Figure 4
Figure 4
(a, b) Recording of responses to increasing concentrations of acetylcholine (10-7-10-3 in control and study groups). (c) Effects of the fructose-rich diet on acetylcholine-evoked (10-7-10-3 M) contractions; ∗∗∗p < 0.001 compared to the control group.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. World Health Statistics. World Health Organization. 2014. http://www.who.int/mediacentre/factsheets/fs311/en/
    1. Reaven G. M. Role of insulin resistance in human disease. Nutrition Journal. 1998 - PubMed
    1. Llewellyn A., Simmonds M., Owen C. G., Woolacott N. Childhood obesity as a predictor of morbidity in adulthood: a systematic review and meta-analysis. Obesity Reviews. 2016;17(1):56–67. doi: 10.1111/obr.12316. - DOI - PubMed
    1. Ulrik C. S. Asthma symptoms in obese adults: the challenge of achieving asthma control. Expert Review of Clinical Pharmacology. 2016;9(1):5–8. doi: 10.1586/17512433.2016.1093415. - DOI - PubMed
    1. Mydlo J. H. Increased intake of protein and fried foods, and female sex seem to increase the risk of renal cancer. The impact of obesity in urology. Urologic Clinics of North America. 2004;31(2):275–287. doi: 10.1016/j.ucl.2004.01.005. - DOI - PubMed

MeSH terms

Substances