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. 2017 Nov 10:21:63-71.
doi: 10.1016/j.nmni.2017.11.001. eCollection 2018 Jan.

Libanicoccus massiliensis gen. nov., sp. nov., a new bacterium isolated from human stool

M Bilen  1   2 , F Cadoret  1 , M Richez  1 , E Tomei  1 , Z Daoud  2 , D Raoult  1   3 , P-E Fournier  1
Affiliations

Libanicoccus massiliensis gen. nov., sp. nov., a new bacterium isolated from human stool

M Bilen et al. New Microbes New Infect. .

Expression of concern in

Abstract

Strain Marseille-P3237 was isolated from a stool sample of a healthy 35-year-old Congolese pygmy female. This anaerobic, Gram-negative, non-spore-forming and non-motile coccus-shaped bacterium is a member of the order Coriobacteriales. It exhibits a 2 009 306-bp genome with a 65.46 mol% G+C content and is closely related to, but distinct from, members of the Olsenella genus. We propose the creation of the new genus Libanicoccus gen. nov. and of the new species Libanicoccus massiliensis sp. nov.

Keywords: Culturomics; Genome; Libanicoccus massiliensis; Pygmy; Taxonogenomics.

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Figures

Fig. 1
Fig. 1
MALDI-TOF MS-generated mass spectrum of Libanicoccus massiliensis gen. nov., sp. nov., strain Marseille-P3237T.
Fig. 2
Fig. 2
Phylogenetic position of Libanicoccus massiliensis strain Marseille-P3237 relative to other closely related species. Phylogenetic relationships were inferred using the maximum-likelihood method. Numbers at the nodes are bootstrap values obtained from 1000 replicates with Shimodaira–Hasegawa test. The scale bar represents a 1% nucleotide sequence divergence.
Fig. 3
Fig. 3
Gel view comparing Libanicoccus massiliensis gen. nov., sp. nov. strain Marseille-P3237T with other closely related species. The gel view displays the raw spectra of loaded spectrum files arranged in a pseudo-gel like look. M/z values are shown on the x axis and the left y-axis represents the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by a Grey scale scheme code. The right y-axis indicates the relation between the colour of a peak and its intensity, in arbitrary units.
Fig. 4
Fig. 4
Gram staining of Libanicoccus massiliensis gen. nov., sp. nov. strain Marseille-P3237T.
Fig. 5
Fig. 5
Transmission electron microscopy of Libanicoccus massiliensis gen. nov., sp. nov. strain Marseille-P3237T.
Fig. 6
Fig. 6
Graphical circular map of the genome of Libanicoccus massiliensis strain Marseille-P3237T. From outside in: contigs (red/grey), COG categories of genes on the forward strand (three circles), genes on the forward strand (blue circle), genes on the reverse strand (red circle), COG categories on the reverse strand (three circles), G+C content.
Fig. 7
Fig. 7
Distribution of functional classes of predicted genes according to the clusters of orthologous groups of proteins.
See this image and copyright information in PMC

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