Anti-inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Induced Mastitis in Mice

Abstract

Rosmarinic acid (RA), a type of food additives mainly extracted from rosemary, has been reported to possess anti-inflammatory activities in some previous studies. However, the effects of RA on lipopolysaccharide (LPS)-induced mastitis have not been reported. Here, we investigated the anti-inflammatory effects of RA on LPS-induced mastitis in mice and elucidated the potential mechanisms in mouse mammary epithelial cells (mMECs). RA treatment significantly ameliorated the mammary structural damage, and reduced the activity of myeloperoxidase. ELISA and qPCR results indicated that RA dose-dependently decreased the expression of TNF-α, IL-1β, and IL-6 both in tissues and mMECs. Furthermore, RA remarkably suppressed the protein levels of TLR4, MyD88, IRAK1, TRAF6, and p-IKKβ. In addition, RA was also found to inhibit LPS-induced NF-κB signaling pathway activation. These results suggest that RA effectively attenuates LPS-induced mastitis by inhibiting the TLR4/MyD88/NF-κB signaling pathway.

Keywords: LPS; NF-κB; TLR4; inflammation; mastitis; rosmarinic acid.