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. 2018 Feb;51(1):e12418.
doi: 10.1111/cpr.12418. Epub 2017 Dec 4.

Circulating microRNA signature of steroid-induced osteonecrosis of the femoral head

Zheng Li  1 , Chao Jiang  1   2 , Xingye Li  1   3 , William K K Wu  4   5 , Xi Chen  1 , Shibai Zhu  1 , Chanhua Ye  1 , Matthew T V Chan  4 , Wenwei Qian  1
Affiliations

Circulating microRNA signature of steroid-induced osteonecrosis of the femoral head

Zheng Li et al. Cell Prolif. 2018 Feb.

Abstract

Objectives: Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association between circulating miRNAs and steroid-induced ONFH remains elusive.

Materials and methods: miRNA microarray was performed to identify the differentially abundant miRNAs in the serums of systemic lupus erythematosus (SLE) patients with steroid-induced ONFH as compared with SLE control and healthy control group. We predicted the potential functions of these differentially abundant miRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and reconstructed the regulatory networks of miRNA-mRNA interactions.

Results: Our data indicated that there were 11 differentially abundant miRNAs (2 upregulated and 9 downregulated) between SLE-ONFH group and healthy control group and 42 differentially abundant miRNAs (14 upregulated and 28 downregulated) between SLE-ONFH group and SLE control group. We also predicted the potential functions of these differentially abundant miRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and reconstructed the regulatory networks of miRNA-mRNA interactions.

Conclusions: These findings corroborated the idea that circulating miRNAs play significant roles in the development of ONFH and may serve as diagnostic markers and therapeutic targets.

Keywords: GO; KEGG; ONFH; circulating microRNAs; osteonecrosis of the femoral head.

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Figures

Figure 1
Figure 1
Differentially abundant miRNAs in serums of SLE‐ONFH patients. A, A heatmap representation of all differentially abundant miRNAs is shown. B, A heatmap of differentially abundant miRNAs between the SLE‐ONFH group and healthy control group. C, A heatmap of differentially abundant miRNAs between SLE‐ONFH group and the SLE control group. D, The heatmap of differentially abundant miRNAs between the SLE and healthy control groups was shown. 1, 2 and 3 denote samples in the SLE‐ONFH group; 112, 114 and 201 denote samples in the SLE control group; 111, 202 and 203 denote healthy control samples
Figure 2
Figure 2
Volcano plots of differentially abundant miRNAs in serums of ONFH patients. A, Volcano plots showing differentially abundant miRNAs between the SLE‐ONFH group and healthy control group. B, Volcano plots showing differentially abundant miRNAs between SLE‐ONFH group and the SLE control group. C, Volcano plots showing differentially abundant miRNAs between the SLE and healthy control groups. Blue and red dots indicate downregulated and upregulated miRNAs, respectively
Figure 3
Figure 3
Functional predictions of differentially abundant miRNAs in SLE‐ONFH vs healthy control. A‐C, Molecular functions, biological processes and cellular components were predicted to be altered by mRNAs targeted by differentially abundant miRNAs in ONFH. Directed Acyclic Graph (DAG) is the graphical display of GO enrichment results with candidate target genes. D, The number of genes in GO term was shown in histograph. E‐G, Enriched biological processes, cellular components and molecular functions in ONFH were shown. H, Scatterplot of enriched KEGG pathway showing statistics of pathway enrichment in ONFH
Figure 4
Figure 4
Functional predictions of differentially abundant miRNAs in SLE‐ONFH vs SLE control. A‐C, Molecular functions, biological processes and cellular components were predicted to be altered by mRNAs targeted by differentially abundant miRNAs in ONFH. Directed Acyclic Graph (DAG) is the graphical display of GO enrichment results with candidate target genes. D, The number of genes in GO term was shown in histograph. E‐G, Enriched biological processes, cellular components and molecular functions in ONFH were shown. H, Scatterplot of enriched KEGG pathway showing statistics of pathway enrichment in ONFH
Figure 5
Figure 5
Functional predictions of differentially abundant miRNAs in SLE vs healthy controls. A‐C, Molecular functions, biological processes and cellular components were predicted to be altered by mRNAs targeted by differentially abundant miRNAs in SLE. Directed Acyclic Graph (DAG) is the graphical display of GO enrichment results with candidate target genes. D, The number of genes in GO term was shown in histograph. E‐G, Enriched biological processes, cellular components and molecular functions were shown. H, Scatterplot of enriched KEGG pathway showing statistics of pathway enrichment in SLE
Figure 6
Figure 6
Regulatory networks of miRNAs and mRNAs between SLE‐ONFH group and healthy control group
Figure 7
Figure 7
Regulatory networks of miRNAs and mRNAs between SLE‐ONFH group and the SLE control group
Figure 8
Figure 8
Regulatory networks of miRNAs and mRNAs between SLE and healthy control groups
See this image and copyright information in PMC

References

    1. Adesina OO, Brunson AM, Gotlib JR, Keegan T, Wun T. Osteonecrosis of the femoral head in sickle cell disease: prevalence, comorbidities and surgical outcomes in California. Blood. 2016;128:22. - PMC - PubMed
    1. Liu XL, Li Q, Sheng JG, et al. Unique plasma metabolomic signature of osteonecrosis of the femoral head. J Orthop Res. 2016;34:1158‐1167. - PubMed
    1. Kuroda Y, Asada R, So K, et al. A pilot study of regenerative therapy using controlled release of recombinant human fibroblast growth factor for patients with pre‐collapse osteonecrosis of the femoral head. Int Orthop. 2016;40:1747‐1754. - PubMed
    1. Okura T, Hasegawa Y, Morita D, Osawa Y, Ishiguro N. What factors predict the failure of curved intertrochanteric varus osteotomy for the osteonecrosis of the femoral head? Arch Orthop Trauma Surg. 2016;136:1647‐1655. - PubMed
    1. Kubo T, Ueshima K, Saito M, Ishida M, Arai Y, Fujiwara H. Clinical and basic research on steroid‐induced osteonecrosis of the femoral head in Japan. J Orthop Sci. 2016;21:407‐413. - PubMed

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