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. 2017 Nov 28;5(1):e422.
doi: 10.1212/NXI.0000000000000422. eCollection 2018 Jan.

Neurofilament light chain predicts disease activity in relapsing-remitting MS

Kristin N Varhaug  1 , Christian Barro  1 , Kjetil Bjørnevik  1 , Kjell-Morten Myhr  1 , Øivind Torkildsen  1 , Stig Wergeland  1 , Laurence A Bindoff  1 , Jens Kuhle  1 , Christian Vedeler  1
Affiliations

Neurofilament light chain predicts disease activity in relapsing-remitting MS

Kristin N Varhaug et al. Neurol Neuroimmunol Neuroinflamm. .

Abstract

Objective: To investigate whether serum neurofilament light chain (NF-L) and chitinase 3-like 1 (CHI3L1) predict disease activity in relapsing-remitting MS (RRMS).

Methods: A cohort of 85 patients with RRMS were followed for 2 years (6 months without disease-modifying treatment and 18 months with interferon-beta 1a [IFNB-1a]). Expanded Disability Status Scale was scored at baseline and every 6 months thereafter. MRI was performed at baseline and monthly for 9 months and then at months 12 and 24. Serum samples were collected at baseline and months 3, 6, 12, and 24. We analyzed the serum levels of NF-L using a single-molecule array assay and CHI3L1 by ELISA and estimated the association with clinical and MRI disease activity using mixed-effects models.

Results: NF-L levels were significantly higher in patients with new T1 gadolinium-enhancing lesions (37.3 pg/mL, interquartile range [IQR] 25.9-52.4) and new T2 lesions (37.3 pg/mL, IQR 25.1-48.5) compared with those without (28.0 pg/mL, IQR 21.9-36.4, β = 1.258, p < 0.001 and 27.7 pg/mL, IQR 21.8-35.1, β = 1.251, p < 0.001, respectively). NF-L levels were associated with the presence of T1 gadolinium-enhanced lesions up to 2 months before (p < 0.001) and 1 month after (p = 0.009) the time of biomarker measurement. NF-L levels fell after initiation of IFNB-1a treatment (p < 0.001). Changes in CHI3L1 were not associated with clinical or MRI disease activity or interferon-beta 1a treatment.

Conclusion: Serum NF-L could be a promising biomarker for subclinical MRI activity and treatment response in RRMS. In clinically stable patients, serum NF-L may offer an alternative to MRI monitoring for subclinical disease activity.

Clinicaltrialsgov identifier: NCT00360906.

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Figures

Figure 1
Figure 1. Levels of neurofilament light chain (NF-L) associated with follow-up time (A) and MRI (B–D)
*p < 0.05.
Figure 2
Figure 2. Levels of chitinase 3-like 1 (CHI3L1) associated with follow-up time (A) and MRI (B–D)
Figure 3
Figure 3. Relationship between new T1 gadolinium-enhanced lesions and time of biomarker measurement
*p < 0.05; **p < 0.005.
See this image and copyright information in PMC

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