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. 2017 Dec 6;10(1):706.
doi: 10.1186/s13104-017-2992-z.

Structural and functional annotation of hypothetical proteins of human adenovirus: prioritizing the novel drug targets

Muhammad Naveed  1   2 , Sana Tehreem  3 , Muhammad Usman  3 , Zoma Chaudhry  3 , Ghulam Abbas  3
Affiliations

Structural and functional annotation of hypothetical proteins of human adenovirus: prioritizing the novel drug targets

Muhammad Naveed et al. BMC Res Notes. .

Abstract

Objective: Human adenoviruses are small double stranded DNA viruses that provoke vast array of human diseases. Next generation sequencing techniques increase genomic data of HAdV rapidly, which increase their serotypes. The complete genome sequence of human adenovirus shows that it contains large amount of proteins with unknown cellular or biochemical function, known as hypothetical proteins. Hence, it is indispensable to functionally and structurally annotate these proteins to get better understanding of the novel drug targets. The purpose was the characterization of 38 randomly retrieved hypothetical proteins through determination of their physiochemical properties, subcellular localization, function, structure and ligand binding sites using various sequence and structure based bioinformatics tools.

Results: Function of six hypothetical proteins P03269, P03261, P03263, Q83127, Q1L4D7 and I6LEV1 were predicted confidently and then used further for structure analysis. We found that these proteins may act as DNA terminal protein, DNA polymerase, DNA binding protein, adenovirus E3 region protein CR1 and adenoviral protein L1. Functional and structural annotation leading to detection of binding sites by means of docking analysis can indicate potential target for therapeutics to defeat adenoviral infection.

Keywords: DNA binding protein; DNA polymerase; DNA terminal protein; Function annotation; Human adenovirus; Hypothetical proteins.

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Figures

Fig. 1
Fig. 1
Flowchart that shows all of the tools used for functional and structural annotation of hypothetical proteins
See this image and copyright information in PMC

References

    1. Davison AJ, Benko M, Harrach B. Genetic content and evolution of adenoviruses. J Gen Virol. 2003;84(11):2895–2908. doi: 10.1099/vir.0.19497-0. - DOI - PubMed
    1. Robinson CM, Singh G, Lee JY, et al. Molecular evolution of human adenoviruses. Sci Rep. 2013;3:1812. doi: 10.1038/srep01812. - DOI - PMC - PubMed
    1. Ramke M, et al. The 5′ UTR in human adenoviruses: leader diversity in late gene expression. Sci Rep. 2017;7(1):618. doi: 10.1038/s41598-017-00747-y. - DOI - PMC - PubMed
    1. Ghebremedhin B. Human adenovirus: viral pathogen with increasing importance. Eur J Microbiol Immunol. 2014;4(1):26–33. doi: 10.1556/EuJMI.4.2014.1.2. - DOI - PMC - PubMed
    1. Scott MK, et al. Human adenovirus associated with severe respiratory infection, Oregon, USA, 2013–2014. Emerg Infect Dis. 2016;22(6):1044. doi: 10.3201/eid2206.151898. - DOI - PMC - PubMed

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