Genome-Wide Association Study of Male Sexual Orientation

Abstract

Family and twin studies suggest that genes play a role in male sexual orientation. We conducted a genome-wide association study (GWAS) of male sexual orientation on a primarily European ancestry sample of 1,077 homosexual men and 1,231 heterosexual men using Affymetrix single nucleotide polymorphism (SNP) arrays. We identified several SNPs with p < 10^-5, including regions of multiple supporting SNPs on chromosomes 13 (minimum p = 7.5 × 10^-7) and 14 (p = 4.7 × 10^-7). The genes nearest to these peaks have functions plausibly relevant to the development of sexual orientation. On chromosome 13, SLITRK6 is a neurodevelopmental gene mostly expressed in the diencephalon, which contains a region previously reported as differing in size in men by sexual orientation. On chromosome 14, TSHR genetic variants in intron 1 could conceivably help explain past findings relating familial atypical thyroid function and male homosexuality. Furthermore, skewed X chromosome inactivation has been found in the thyroid condition, Graves' disease, as well as in mothers of homosexual men. On pericentromeric chromosome 8 within our previously reported linkage peak, we found support (p = 4.1 × 10^-3) for a SNP association previously reported (rs77013977, p = 7.1 × 10^-8), with the combined analysis yielding p = 6.7 × 10^-9, i.e., a genome-wide significant association.

Figure 1

Manhattan Plot of GWAS for Male Sexual Orientation. Plot of negative log₁₀ of the p-values for the single SNP association analysis of 1,077 homosexual and 1,231 heterosexual men, ordered along the x-axis for each chromosome by chromosomal position.