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. 2017 Dec 12;12(1):189.
doi: 10.1186/s13018-017-0679-8.

Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats

Shun Lyu  1 , Bin Ji  1 , Wenwu Gao  1 , Xianqi Chen  1 , Xiaotao Xie  1 , Junjie Zhou  2
Affiliations

Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats

Shun Lyu et al. J Orthop Surg Res. .

Abstract

Background: Knee osteoarthritis (KOA) is a common orthopedics disease and its pathological changes at early stage are the damage and loss of articular cartilage. Traditional Chinese medicine (TCM) prescription contains multiple components and has the unique advantages of the diversity of targets.We compared the traditional Chinese medical formulae (Angelicae Pubescentis and Loranthi decotion, APLD, or Duhuo Jisheng) with a western medicine (glucosamine sulfate, GS) to treat the rat arthritis models, and tracked the outcomes.

Methods: Thirty-two Wistar rats (weight 180 ± 10 g, 6-week-old) were randomly divided into four groups (eight for each): group A as normal control group (no surgery and no drug treatment), group B as SIA (surgery-induced arthritis) model control without drug treatment, group C as SIA model + APLD, and group D as SIA model + GS. Anterior cruciate ligament in the knee joint of both hind legs from each rat in groups B, C, and D was shown and cut off to establish the SIA model. After 6 weeks of the surgery, rats in group C or D were treated with APLD or GS, respectively, for 8 weeks. Bone X-ray examination, histological images, and determination of genes of collagen II and aggrecan were performed. At week 14, both knee joint gap and bone structure disappeared in rats of group B, but they were visible in rats of groups A, C, and D.

Results: Histological images revealed that the structure and composition of the knee joint cartilage were significantly degenerated in group B and improved in group C. Genes of collagen II and aggrecan were significantly increased in both group C and D.

Conclusion: APLD or GS gavage treatment for knee osteoarthritis (KOA) rat models was effective on the proliferation of cartilage chondrocytes and the damaged knee joint tissue repairing, and the APLD showed slightly superior in general.

Keywords: Angelicae Pubescentis and Loranthi decotion; Chondrocyte; Duhuo; Knee osteoarthritis; Serum.

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Conflict of interest statement

Ethics approval

The study protocol was approved by the Ethics Committee of the Shanghai University of Traditional Chinese Medicine.

Consent for publication

The current research did not involve human participants, this information is not applicable.

Competing interests

The authors declare that they have no competing interests.

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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
The X-ray images of the right hind leg of rats from each group were taken at 6, 10 and 14 weeks, respectively. A, E, I belong to group A (blank, control group, no surgery and no drug treatment); B, F, J belong to group B (the SIA model control without drug treatment); C, G, K belong to group C (the SIA model + APLD treatment); D, H, L belong to group D (the SIA model + GS treatment)
Fig. 2
Fig. 2
Right knee articular cartilage of histological imaging with staining of H&E, toluidine blue, and collagen II immunohistochemistry at weeks 10 and 14, which meant after medicine gavage treatment (4 and 8 weeks, respectively). A–H with H&E staining; I-P with toluidine blue staining; and Q–X with collagen II immunohistochemistry staining. Scale bar was 100 μm. Blank: group A; saline: group B; APLD: group C; and GS: group D
See this image and copyright information in PMC

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