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. 2017 Nov 21:16:327-333.
doi: 10.1016/j.dib.2017.11.058. eCollection 2018 Feb.

Data supporting that adipose-derived mesenchymal stem/stromal cells express angiotensin II receptors in situ and in vitro

Liudmila V Ageeva  1 , Veronika Y Sysoeva  1 , Pyotr A Tyurin-Kuzmin  1 , George V Sharonov  1 , Daniyar T Dyikanov  1 , Dmitry V Stambolsky  1 , Natalia I Kalinina  1
Affiliations

Data supporting that adipose-derived mesenchymal stem/stromal cells express angiotensin II receptors in situ and in vitro

Liudmila V Ageeva et al. Data Brief. .

Abstract

This article contains results of analyses of angiotensin II receptors expression in human adipose tissue and stem/stromal cells isolated from adipose tissue. We also provide here data regarding the effect of angiotensin II on intracellular calcium mobilization in adipose tissue derived stem/stromal cells (ADSCs). Discussion of the data can be found in (Sysoeva et al., 2017) [1].

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Figures

Fig. 1
Fig. 1
Immunofluorescent staining of frozen adipose tissue sections with antibodies against type 1 (A.) and type 2 (B.) angiotensin receptor (green fluorescence) and ADSCs marker PDGFRbeta (green fluorescence). Scale bars on left panels correspond to 100 µm, on right panels - to 50 µm. Nuclei are counterstained with DAPI (blue fluorescence).
Fig. 2
Fig. 2
Immunofluorescent staining of frozen adipose tissue sections with antibodies against type 1 angiotensin receptor (red fluorescence) and endothelial cell marker CD31 (green fluorescence). Scale bar on the left panel corresponds to 100 µm, on right panels - to 20 µm. Nuclei are counterstained with DAPI (blue fluorescence).
Fig. 3
Fig. 3
Immunofluorescent staining of frozen adipose tissue sections with antibodies against MAS1 receptor (red fluorescence). The scale bar corresponds to 50 µm. Nuclei are counterstained with DAPI (blue fluorescence).
Fig. 4
Fig. 4
Representative image (A.) and recordings (B.) of intracellular Ca2+ transients in individual ADSCs, treated with Ang II (10 nM) in series in the presence of AT1 receptor antagonist losartan. Short lines above fluorescence trace show applications of indicated compounds. ∆F/F0 = 1 – fluorescence level of the cell without treatment.
Fig. 5
Fig. 5
Expression of AT1 receptor on cultured ADSCs. Representative flow cytometry plots. Staining at room temperature. A- IgG-APC, IgG-DyLight-488. B – antibodies against AT1 receptor.
Fig. 6
Fig. 6
Expression of AT1 receptor on cultured ADSCs. Representative flow cytometry plots. Staining at room temperature. A. – AT2 receptor staining. B. – Double staining of AT1 and AT2 receptors. C. – AT2 expression in ADSCs stably expressing AT1. AT1+-cells are shown only, AT1 receptor individual staining – gray curve, AT1 and AT2 receptor double staining – blue curve. Flow cytometry analysis of ADSCs, stained with antibodies against AT2 receptor.
See this image and copyright information in PMC

References

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