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Comparative Study
. 2018 Jul-Sep;49(3):656-661.
doi: 10.1016/j.bjm.2017.03.018. Epub 2017 Dec 6.

Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods

Jorge Enrique Wong Paz  1 , Carolina Rubio Contreras  2 , Abigail Reyes Munguía  2 , Cristóbal Noé Aguilar  3 , María Luisa Carrillo Inungaray  4
Affiliations
Comparative Study

Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods

Jorge Enrique Wong Paz et al. Braz J Microbiol. 2018 Jul-Sep.

Abstract

Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S. paratyphi, and by the Minimum Bactericidal Concentration, the percentage of activity and the Index of Bacterial Susceptibility of each extract. The phenolic compound identified in different concentrations in the three extracts was epicatechin. The extracts obtained by the three methods had antimicrobial activity, however, there was no significant difference (p<0.05) between the Minimum Bactericidal Concentration of the extracts obtained by maceration, percolation and Soxhlet. The results of this study contribute to the body of knowledge on the use of extracts in controlling microorganisms with natural antimicrobials.

Keywords: Antibacterial activity; HPLC–MS; Hamelia patens; Polyphenols.

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Figures

Fig. 1
Fig. 1
HPLC chromatogram of extracts of leaves Hamelia patens obtained by percolation (A), Soxhlet (B) and maceration (C).
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