Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Apr-Jun;30(2):77-82.
doi: 10.1590/0102-6720201700020001.

PERITONEAL ADHESIONS TYPE I, III AND TOTAL COLLAGEN ON POLYPROPYLENE AND COATED POLYPROPYLENE MESHES: EXPERIMENTAL STUDY IN RATS

[Article in English, Portuguese]
Lucas Félix Rossi  1 , Manoel Roberto Maciel Trindade  1 , Armando José D Acampora  1 , Luise Meurer  1
Affiliations

PERITONEAL ADHESIONS TYPE I, III AND TOTAL COLLAGEN ON POLYPROPYLENE AND COATED POLYPROPYLENE MESHES: EXPERIMENTAL STUDY IN RATS

[Article in English, Portuguese]
Lucas Félix Rossi et al. Arq Bras Cir Dig. 2017 Apr-Jun.

Abstract
in English, Portuguese

Background: Hernia correction is a routinely performed treatment in surgical practice. The improvement of the operative technique and available materials certainly has been a great benefit to the quality of surgical results. The insertion of prostheses for hernia correction is well-founded in the literature, and has become the standard of treatment when this type of disease is discussed.

Aim: To evaluate two available prostheses: the polypropylene and polypropylene coated ones in an experimental model.

Methods: Seven prostheses of each kind were inserted into Wistar rats (Ratus norvegicus albinus) in the anterior abdominal wall of the animal in direct contact with the viscera. After 90 days follow-up were analyzed the intra-abdominal adhesions, and also performed immunohistochemical evaluation and videomorphometry of the total, type I and type III collagen. Histological analysis was also performed with hematoxylin-eosin to evaluate cell types present in each mesh.

Results: At 90 days the adhesions were not different among the groups (p=0.335). Total collagen likewise was not statistically different (p=0.810). Statistically there was more type III collagen in the coated polypropylene group (p=0.039) while type I was not different among the prostheses (p=0.050). The lymphocytes were statistically more present in the polypropylene group (p=0.041).

Conclusion: The coated prosthesis was not different from the polypropylene one regarding the adhesion. Total and type I collagen were not different among the groups, while type III collagen was more present on the coated mesh. There was a greater number of lymphocytes on the polypropylene mesh.

Racional:: A correção herniária é tratamento realizado rotineiramente na prática cirúrgica. O aprimoramento da técnica operatória e dos materiais disponíveis trouxe grande benefício na qualidade dos resultados cirúrgicos. A inserção de próteses para correção herniária é bem embasada na literatura e tornou-se o padrão de tratamento.

Objetivo:: Avaliar em modelo experimental dois tipos de próteses diferentes, de polipropileno e polipropileno revestido.

Métodos:: Foram inseridas sete próteses de cada tipo em ratos Wistar (Ratus norvegicus albinus) na parede abdominal anterior do animal em contato direto com as vísceras. Após o seguimento de 90 dias analisaram-se as aderências intra-abdominais, bem como avaliação por imunoistoquímica e videomorfometria do colágeno total, tipo I e tipo III. Também, fez-se análise histológica com hematoxylina-eosina para avaliação dos tipos celulares presentes em cada tela.

Resultados:: Aos 90 dias as aderências não foram diferentes entre os grupos (p=0,335). O colágeno total igualmente não foi estatisticamente diferente (p=0,810). O colágeno tipo III foi estatisticamente maior no grupo polipropileno revestido (p=0,039) enquanto o tipo I não diferiu entre as próteses (p=0,050). Os linfócitos foram estatisticamente mais presentes no grupo polipropileno (p=0,041).

Conclusão:: A prótese revestida não foi diferente da de polipropileno na variável aderência. O colágeno total e tipo I não foram diferentes entre os grupos enquanto que o colágeno tipo III foi mais presente na tela revestida. O número de linfócitos foi maior na tela de polipropileno.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest: none

Figures

FIGURE 1
FIGURE 1. Insertion of prostheses, on the left the polypropylene mesh and its fixation with polypropylene 4-0 suture. On the right, mesh that separates tissues, fixed with the same suture.
FIGURE 2
FIGURE 2. Resected anterior abdominal wall with the implanted prosthesis - polypropylene group
FIGURE 3
FIGURE 3. Process of videomorphometric analysis: collagen stained with picrosirius and quantitatively evaluated by number of pixels in the digitized image
FIGURE 4
FIGURE 4. Colon and omentum adhesion in the Proceed group, notably more localized at the insertion of the mesh to the wall
FIGURE 5
FIGURE 5. Forest graph showing the differences of standardized means (S/S), among the variable selected for the group treated with the coated prosthesis and the polypropylene one
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Andreollo NA, Brandalise NA, Fagundes JJ, Mantovani M, Leonardi LS. Hérnias incisionais avaliação do tratamento cirúrgico. Revista Paulista de Medicina. 1984;102(1):23–26. - PubMed
    1. Balbinotto RP, Muller AL, Nunes AG, Da Silva R, Meyer FS, Cerski CS. Barrier methods used to prevent pelvic adhesions in videolaparoscopy experimental study in female rabbits. Surgical endoscopy. 2011;25(8):2637–2642. - PubMed
    1. Cassar K, Munro A. Surgical treatment of incisional hernia. The British journal of surgery. 2002;89(5):534–545. - PubMed
    1. Chan G, Chan CK. A review of incisional hernia repairs preoperative weight loss and selective use of the mesh repair. Hernia : the journal of hernias and abdominal wall surgery. 2005;9(1):37–41. - PubMed
    1. Costa RGd, Lontra MB, Scalco P, Cavazzola LT, Gurski RR. Polylactic acid film versus acellular porcine small intestinal submucosa mesh in peritoneal adhesion formation in rats. Acta cirurgica brasileira. 2009;24:128–135. - PubMed