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. 2018 Feb 8;131(6):703-706.
doi: 10.1182/blood-2017-08-803031. Epub 2017 Dec 19.

Normal plasma IgG inhibits HIT antibody-mediated platelet activation: implications for therapeutic plasma exchange

Curtis G Jones  1 , Shannon M Pechauer  2 , Brian R Curtis  3 , Daniel W Bougie  2 , Richard H Aster  2   4 , Anand Padmanabhan  1   2   5
Affiliations

Normal plasma IgG inhibits HIT antibody-mediated platelet activation: implications for therapeutic plasma exchange

Curtis G Jones et al. Blood. .
No abstract available

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Conflict of interest statement

Conflict-of-interest disclosure: C.G.J., D.W.B., R.H.A., and A.P. state that a patent application has been filed related to diagnostic testing in HIT (Method of Detecting Platelet-Activating Antibodies That Cause Heparin-Induced Thrombocytopenia/Thrombosis; PCT/US14/62591). The remaining authors declare no competing financial interests.

Figures

Figure 1.
Figure 1.
Normal plasma inhibits HIT-1–mediated platelet activation more effectively than 5% albumin. (A-B) PEA test results are depicted as a percentage of the value obtained with undiluted HIT-1. Red and blue bars show results obtained with HH131 and RR131 FcγRIIa platelets, respectively. Results obtained with 5% albumin dilution are represented by the horizontal dotted (RR131 platelets) and solid (HH131 platelets) lines. Comparable results were obtained with plasma from 3 different normal individuals (normal plasma #1-#3). Means +1 standard deviation of triplicate determinations are presented and were compared by using the Student t test. P < .05 was considered significant. *P < .05; **P < .01; ***P < .001. ns, not significant.
Figure 2.
Figure 2.
IgG-repleted normal plasma inhibits platelet activation induced by HIT-1 more effectively than IgG-depleted plasma. (A-B) The abscissa indicates dilution ratio of HIT-1 with IgG-depleted and -repleted samples obtained from 3 different normal plasmas (normal plasma #1-#3), and the ordinate depicts the PEA as a percentage of the value obtained with undiluted HIT-1. FcγRIIa genotype is indicated on the abscissa. Red and blue bars represent HIT-1 diluted with IgG-depleted and -repleted normal plasma, respectively. (C) IgG status of the diluent has little to no effect on results obtained with HIT-1 dilution in the PF4 enzyme-linked immunosorbent assay (ELISA). The abscissa indicates dilution ratio used, and the ordinate depicts the optical density (OD) of IgG-specific PF4-polyvinylsulfonate ELISA (PF4 ELISA). All PF4 ELISA reactions (ODs) were inhibited ≥50% with high-dose (100 U/mL) heparin (data not shown). Means +1 SD of triplicate determinations are presented and were compared by using the Student t test. P < .05 was considered significant. *P < .05; **P < .01; ***P < .001; ****P < .0001.
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References

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