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. 2017 Dec 6:10:463-467.
doi: 10.2147/IDR.S147272. eCollection 2017.

Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis

Pilar Ruiz  1 , Manuel Causse  2 , Manuel Vaquero  1 , Juan Bautista Gutierrez  1   2 , Manuel Casal  1   2
Affiliations

Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis

Pilar Ruiz et al. Infect Drug Resist. .

Abstract

A new automated real-time PCR assay for the detection of rifampicin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB) was evaluated. A total of 163 clinical samples (128 pulmonary and 35 extra-pulmonary) were processed using four PCR assay kits: Abbott RealTime MTB RIF/INH, Genotype MTBDRplus, Xpert/MTB RIF, and Anyplex MTB/MDR. The results of phenotypic drug-susceptibility testing using BACTECMGIT 960 were used as reference. The sensitivity and specificity of the new Abbott RealTime MTB RIF/INH assay in comparison with phenotypic testing was 96.3% (95%CI 87.32%-100%) for RIF and 100% (95%CI 99.3%-100%) for INH; the sensitivity was 78.8% (95%CI 66.8%-90.9%) and the specificity was 100% (95%CI 98.9%-100%). The Abbott RealTime MTB RIF/INH test could be a valid method for detecting the most common mutations in strains resistant to RIF and INH.

Keywords: Abbott RealTime MTB RIF/INH Resistance assay; M. tuberculosis; mutations.

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Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Flow diagram for Abbott RealTime MTB RIF/INH evaluation. Abbreviations: MTB, Mycobacterium tuberculosis; RIF, rifampicin; INH, isoniazid; LOD, level of detection; DST, drug-susceptibility testing.
See this image and copyright information in PMC

References

    1. World Health Organization . Guidelines for surveillance of drug resistance in tuberculosis. Geneva, Switzerland: WHO; 2016. 2016. [Accessed November 21, 2017]. Available from: http://apps.who.int/iris/bitstream/10665/174897/1/9789241549134_eng.pdf?....
    1. World Health Organization . Global tuberculosis report 2016. Geneva, Switzerland: WHO; 2016. [Accessed November 21, 2017]. Available from: http://apps.who.int/medi-cinedocs/documents/s23098en/s23098en.pdf.
    1. World Health Organization, 2016 . The use of molecular line probe assays for the detection of resistance to isoniazid and Rifampicin: Policy update. Geneva, Switzerland: WHO; 2016. [Accessed November 21,2017]. Available from: http://apps.who.int/iris/bitstream/10665/250586/1/9789241511261-eng.pdf?....
    1. European Center for Disease Prevention and Control/WHO Regional Office for Europe . Tuberculosis surveillance and monitoring in Europe, 2017. ECDC; 2017. [Accessed November 21, 2017]. Available from: https://ecdc.europa.eu/sites/portal/files/media/en/publications/Publicat....
    1. Rusch-Gerdes S, Pfyffer GE, Casal M, Chadwick M, Siddiqi S. Multicenter laboratory validation of the BACTEC MGIT 960 technique for testing susceptibilities of Mycobacterium tuberculosis to classical second-line drugs and newer antimicrobials. J Clin Microbiology. 2006;44(3):688–692. - PMC - PubMed