Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec 22;7(1):18092.
doi: 10.1038/s41598-017-18333-7.

Diagnosis and typing of influenza using fluorescent barcoded probes

Bixing Huang  1 , Nicholas P West  2 , Jelena Vider  2 , Amanda J Cox  2 , Tanya Constantino  1 , Bruce J Harrower  1 , Alyssa T Pyke  1 , Jamie McMahon  1 , Judith A Northill  1 , Tim Riordan  3 , David Warrilow  4
Affiliations

Diagnosis and typing of influenza using fluorescent barcoded probes

Bixing Huang et al. Sci Rep. .

Abstract

In this work, we explore a new hybridization technology using barcoded probes which has large-scale multiplexing capability. We used influenza virus to test whether the technology has application in virus diagnostics. Typing of influenza virus strains is an important aspect of global health surveillance. Standard typing procedures use serological or amplification-based assays performed sequentially. By comparison, the hybridization technology was correctly able to detect, type and subtype influenza A and B virus strains directly from clinical samples in a single reaction without prior virus isolation or amplification. Whilst currently not as sensitive as amplification-based assays, these results are a first-step towards application of this technology to the detection and typing of influenza and other viruses.

PubMed Disclaimer

Conflict of interest statement

T.R. is an employee of NanoString Technologies. Otherwise, the authors have no competing financial interests to declare.

Figures

Figure 1
Figure 1
Detection, typing, and subtyping of influenza virus isolates. RNA extracts of influenza isolates were (A) detected and typed; and simultaneously sub-typed as strains of (B) influenza A or (C) influenza B. (D) A new probe based on PB1 sequence (probe 7) for the detection of influenza A is shown in comparison to the original MP probe (probe 1).
Figure 2
Figure 2
Determination of the linear range of the FluST assay. A series of six 10-fold dilutions of each of an isolate previously typed and sub-typed as either (A) A-H1N1pdm09 strain or (B) B-Victoria strain. Counts from both typing and sub-typing probes are given for each isolate.
See this image and copyright information in PMC

References

    1. World Health Organization. Influenza update no. 294, http://www.who.int/influenza/surveillance_monitoring/updates/2017_07_24_... (2017).
    1. Le TH, Nguyen NT. Evolutionary dynamics of highly pathogenic avian influenza A/H5N1 HA clades and vaccine implementation in Vietnam. Clin Exp Vaccine Res. 2014;3:117–127. doi: 10.7774/cevr.2014.3.2.117. - DOI - PMC - PubMed
    1. Minosse C, et al. Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR. J Virol Methods. 2007;141:225–228. doi: 10.1016/j.jviromet.2006.12.010. - DOI - PMC - PubMed
    1. Song MK, Chang J, Hong Y, Hong S, Kim SW. Direct multiplex reverse transcription-nested PCR detection of influenza viruses without RNA purification. J Microbiol Biotechnol. 2009;19:1470–1474. - PubMed
    1. Geiss GK, et al. Direct multiplexed measurement of gene expression with color-coded probe pairs. Nat Biotechnol. 2008;26:317–325. doi: 10.1038/nbt1385. - DOI - PubMed

Publication types